Abstract
Chemotaxis is directed migration of a cell type to a distant chemoattractant. When this chemoattractant is a cytokine, the term chemokine is often used. Chemotaxis by neutrophils, specifically polymorphonuclear leukocytes (PMNs), plays a critical role in the innate immune response. On an equimolar basis, interleukin-8 (IL-8) is one of the most potent PMN chemokines known. This chapter describes an in vitro chemotaxis technique using PMNs and IL-8 which can serve the investigator as an established model from which new studies can be developed.
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© 2014 Springer Science+Business Media, New York
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Davidson, D., Patel, H. (2014). Cytokine-Induced Neutrophil Chemotaxis Assay. In: Vancurova, I. (eds) Cytokine Bioassays. Methods in Molecular Biology, vol 1172. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0928-5_9
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DOI: https://doi.org/10.1007/978-1-4939-0928-5_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0927-8
Online ISBN: 978-1-4939-0928-5
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