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Purification of a nuclease from human serum

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Summary

The purification procedure for a nuclease from human serum is described. It includes ammonium sulfate precipitation, chromatography on DEAE-Sephadex and on Sephacryl-S 200, and preparative electrophoresis. The enzyme, purified about 2000-fold, is homogeneous in a sodium dodecyl sulfate electrophoretic system, where it has a mol. wt of 78,000. The pH optimum lies around pH 6.5; it is a sugar-nonspecific endonuclease.

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Author notes

  1. G. Seibert

    Present address: Hoechst AG, D-6230, Frankfurt/Main 80, FRG

Authors and Affiliations

  1. Physiologisch-Chemisches Institut der Johannes-Gutenberg-Universität, D-6500, Mainz, Federal Republic of Germany

    E. J. Zöllner, G. Seibert, H. Slor & R. K. Zahn

  2. Department of Human Genetics, Tel Aviv University, Tel Aviv, Israel

    E. J. Zöllner, G. Seibert, H. Slor & R. K. Zahn

Authors
  1. E. J. Zöllner
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  2. G. Seibert
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  3. H. Slor
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  4. R. K. Zahn
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Additional information

Acknowledgment. This work has been supported by a research grant from Stiftung Volkswagenwerk through the Akademie der Wissenschaften und der Literatur, Mainz. We thank Mrs R. Nehrbass and Mrs C. Wolpert for technical assistance.

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Zöllner, E.J., Seibert, G., Slor, H. et al. Purification of a nuclease from human serum. Experientia 37, 548–550 (1981). https://doi.org/10.1007/BF01990041

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