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In vitro andin vivo effects of gold salts on chemotaxis and random migration of rat polymorphonuclear leucocytes

  • Immunosuppression and Inflammation
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Abstract

The effect produced by three gold salts (sodium aurothiomalate, allochrysine, auranofin) on chemotaxis and random migration of rat polymorphonuclear leucocytes (PMN) was investigated under various experimental conditions.

The drug activity was examined after incubationin vitro or after administrationin vivo.

PMNs were recruited after the induction of two acute inflammatory reactions (pleurisies induced by isologous serum or a suspension of calcium pyrophosphate (CaPP) crystals).

The three gold salts administeredin vivo andin vitro inhibited the chemotactic responses of the two cell types. This action was dose-dependent. Auranofin was the most effective substance while sodium aurothiomalate was the least.

The random migration was not always significantly depressed especially for CaPP-elicited cells.

Reduction in neutrophil chemotaxis might be an important additional mechanism in the action of gold salts and their activity on inflammatory PMNs recruited at inflammatory foci might be beneficial in the treatment in rheumatic diseases in which PMN migration would be implicated.

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References

  1. S. Boyden,The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes, J. exp. Med.115, 453–466 (1962).

    Article  PubMed  Google Scholar 

  2. T.D. Coates, B. Wolach, D.Y. Tzeng, G. Higgins, R.L. Baehner andL.A. Boxer,The mechanism of action of the antiinflammatory agents dexamethasone and auranofin in human PMN leukocytes. Blood62, 1070–1077 (1983).

    PubMed  Google Scholar 

  3. P. Davis, C. Johnston, C.L. Miller andK. Wong,Effects of gold compounds on the function of phagocytic cells. II.Inhibition of superoxide radical generation by tripeptide-activated PMN leukocytes, Arthiritis Rheum.36, 82–86 (1983).

    Google Scholar 

  4. R.S. Ennis, J.L. Granda andA.S. Posner,Effect of gold salts and other drugs on the release and activity of lysosomal hydrolases, Arthritis Rheum.11, 756–764 (1968).

    PubMed  Google Scholar 

  5. J. Forestier,Rheumatoid arthritis and its treatment by gold salts: results of six years experiments. J. clin. Lab. clin. Med.20, 827–840 (1935).

    Google Scholar 

  6. P. Galle andJ. Villiaumey,Concentration de l'or par les macrophages de la moelle osseuse au cours d'affections rhumatismales soumises à la chrytothérapie, Revue du Rhumatisme49, 273–279 (1982).

    Google Scholar 

  7. J.P. Giroud, M. Roch-Arveiller andO. Muntaner,Prélèvement répété des polynucléaires dans la cavité pleurale du rat. Application à l'étude du chimiotactisme, Nelle Rev. Fse Hémat.20, 535–543 (1978).

    Google Scholar 

  8. H.U. Keller, H. Gerber, M.W. Hess andH. Cottier, Studies on the regulation of the neutrophil chemotactic response using a rapid and reliable method for measuring random migration and chemotaxis of neutrophil granulocytes. InFuture Trends in Inflammation II, pp. 326–339 (EdsJ.P. Giroud, D.A. Willoughby andG.P. Velo). Birkhäuser Verlag, Basel and Stuttgart 1975.

    Google Scholar 

  9. H.U. Keller, P.C. Wilkinson, M. Abercrombie, E.L. Becker, J.G. Hirsch, M.E. Miller, W. Ramsey andS.H. Zigmond,A proposal for the definition of terms related to locomotion of leucocytes and other cells, Cell biol. Intern. Reports1, 391–397 (1977).

    Article  Google Scholar 

  10. S.T. Lamprecht, A.E. Finkelstein, D.T. Walz andJ. Kraviec,Inhibition of PGE 1-stimulation of cyclic AMP formation by auranofin. Comm. 15th Congress of rheumatol. Paris (1981).

  11. J.H. Leibfarth andR.H. Persellin,Review: mechanisms of action of gold, Agents and Actions11 (5), 458–472 (1981).

    PubMed  Google Scholar 

  12. A.J. Lewis, J. Cottney, D.D. White, P.K. Fox, A. McNeillie, J. Dunlop, W.E. Smith andD.H. Brown,Action of gold salts in some inflammatory and immunological models, Agents and Actions10, 63–70 (1980).

    PubMed  Google Scholar 

  13. A.G. Mowat,Neutrophil chemotaxis in rheumatoid arthritis drug effects. Ann. Rheum. Dis.36 (3), 287 (1977).

    Google Scholar 

  14. A.G. Mowat,Neutrophil chemotaxis in rheumatoid arthritis: effect of d-penicillamine, gold salts and levamisole, Ann. rheum. Dis.37, 1–8 (1978).

    PubMed  Google Scholar 

  15. A. Pecoud, A. Leimgruber andP.C. Frei,Effet de substances anti-inflammatoires sur le chimiotactisme des polynucléaires humains étudié in vitro, Schweiz. med. Wschr.108, 1969–1970 (1978).

    PubMed  Google Scholar 

  16. A. Pecoud, A. Leimgruber andP.C. Frei,Effect of one gold salt, of betamethasone, and of aspirin on the chemotaxis of human neutrophils measured in vitro,Ann. rheum. Dis. 30, 25–30 (1980).

    Google Scholar 

  17. N.O. Rothermich, Chrysotherapy in rheumatoid arthritis. InAntirheumatic Drugs (Ed.E.C. Huskisson). pp. 509–520 CPTS (3), Praeger 1983.

  18. E. Schiffmann, B.A. Corcoran andS.M. Wahl,N-formyl propionyl peptides as chemoattractants for leucocytes, Proc. natl. Acad. Sci. USA72, 1059–1062 (1975).

    PubMed  Google Scholar 

  19. H.J. Showell, R.J. Freer, S.J. Zigmond, E. Schiffmann, S. Aswanikumar, B. Corcoran andE.L. Becker The structure activity relations of synthetic peptides as chemotactic factors and inducers of peptides as chemotactic factors and inducers of lysosomal enzyme secretion for neutrophils, J. exp. Med.143, 1154–1169 (1978).

    Article  Google Scholar 

  20. R.A. Turner, J.A. Johnson andE.L. Semble,Antirheumatic drug effects on neutrophil response to chemotactic factors: a comparison of analytical techniques, Proc. Soc. Exp. Biol. Med.173, 200–204 (1983).

    PubMed  Google Scholar 

  21. B. Vernon-Roberts,Action of gold salts on the inflammatory response and inflammatory cell function, J. Rheumatol.6 (suppl. 5), 120–129 (1979).

    Google Scholar 

  22. D.A. Willoughby, C.J. Dunn, S. Yamamoto, F. Capasso, D.A. Deporter andJ.P. Giroud,Calcium pyrophosphate-induced pleurisy in rat: a new model of acute inflammation, Agents and Actions5, 35–38 (1975).

    PubMed  Google Scholar 

  23. S.H. Zigmond andJ.G. Hirsch,Leukocyte locomotion and chemotaxis. New methods for evaluation and demonstration of a cell derived chemotactic factor, J. exp. Med.137, 387–410 (1973).

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology, CNRS ERA 629, Hopital Cochin, 27, rue du Faubourg Saint-Jacques, 75014, Paris, France

    D. Pham Huy, M. Roch-Arveiller, O. Muntaner & J. P. Giroud

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  1. D. Pham Huy
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  2. M. Roch-Arveiller
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  3. O. Muntaner
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  4. J. P. Giroud
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Pham Huy, D., Roch-Arveiller, M., Muntaner, O. et al. In vitro andin vivo effects of gold salts on chemotaxis and random migration of rat polymorphonuclear leucocytes. Agents and Actions 16, 363–368 (1985). https://doi.org/10.1007/BF01982874

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  • DOI: https://doi.org/10.1007/BF01982874

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