Abstract
The action of the polyamines compound 48/80, poly-1-lysine and polymyxin B on rat pleural and peritoneal mast cell secretion have been studied. Unpurified pleural mast cells released more histamine than peritoneal mast cells when stimulated by submaximal concentrations of compound 48/80 and poly-1-lysine, but the same profile of response was observed with polymyxin B in both populations. Doseresponse studies of peritoneal and pleural mast cells purified with Percoll and Ficoll and stimulated by polymxin B showed a decreased sensitivity and decreased maximum response of peritoneal cells when Percoll was used. The maximal response of pleural cells and the sensitivity of peritoneal cells were affected only sglightly by Ficoll.
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References
D. Lagunoff andT.W. Matrin,Agents that release histamine from mast cells, Ann. Rev. Pharmac. Toxic.23, 335–351 (1983).
G.W. Read, S.M. Hong andE.F. Kiefer,Competitive inhibition of 48/80-induced histamine release by benzalkonium chloride and its analogs and the polyamine receptor in mast cells, J. Pharmac. exp. Ther.222, 652–657 (1982).
W. Kazimierczac andB. Diamant, Mechanisms of histamine release in anaphylactic and anaphylactoid reactions. InProgress in Allergy, vol. 24, pp. 295–365 (Eds.P. Kallós et al.), S. Karger, Basel 1978.
N. Grosman andB. Diamant,Binding of 45 calcium to isolated rat mast cells in connection with histamine release, Agents and Actions,8, 338–346 (1978).
A. Sydbom, B. Fredholm andB. Uvnas,Evidence against a role of cyclic nucleotides in the regulation of anaphylactic histamine release in isolated rat mast cells, Acta Physiol. Scand.112, 47–56 (1981).
J. Biemenstock, A.D. Befus, J. Demburg, R. Goodacre, F. Pearce andF. Shanagan, Mast cell heterogeneity. InMonographs in Allergy, vol. 18, pp. 124–128 (Eds.P. Kallós et al.), S. Karger, Basel 1983.
J. Espinosa, L.M. Botana andP. Fernández-Otero,Estudio de la actividad secretora de mastocitos en presencia de agonistas colinérgicos, Rev. Esp. Fisiol.39, 441–446 (1983).
L. Enerback andI. Svenson,Isolation of rat peritoneal mast cells by centrifugation on density gradients of Percoll, J. Immunol. Methods39, 135–145 (1980).
K.E. Barret andF.L. Pearce,A comparative study of histamine secretion from rat peritoneal and pleural mast cells, Agents and Actions12, 186–188 (1982).
K.W. Batchelor andD.R. Stanworth,A comparison of the histamine-releasing properties of rat pleural and peritoneal mast cells., Immunology41, 271–278 (1980).
A.R. Johnson andN.C. Moran,Release of histamine from rat mast cells: a comparison of the effects of 48/80 and two antigen antibody systems, Fed. Proc.28, 1716–1720 (1969).
P.T. Peachell andF.L. Pearce,Some studies on the release of histamine from rat mast cells treated with polymyxin B, Agents and Actions14, 379–385 (1984).
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Botana, L.M., Espinosa, J., Eleno, N. et al. Difference in, an influence of the purification medium on, sensitivity and maximum response of peritoneal and pleural mast cells stimulated by certain polyamines. Agents and Actions 16, 342–345 (1985). https://doi.org/10.1007/BF01982870
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DOI: https://doi.org/10.1007/BF01982870