Skip to main content
SpringerLink
Log in

Modulation of the effect of histamine-releasing lymphokine on human basophils

  • Histamine Release and Pathological States
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

Agents which increase or mimic intracellular cyclic 3′,3′-adenosine monophosphate (cAMP)-theophylline dibutyryl cAMP (DBcAMP) and isoprenaline (in the presence of theophylline)-all produced pronounced inhibition of histamine release from human basophils, thus suggesting a regulatory role for the cAMP system. The effect of the flavonoids, quercetin and taxifolin, and the structurallyrelated cromone disodium cromoglycate (DSCG) was also studied. Only quercetin was effective in inhibiting histamine release. This is similar to the situation in IgE-mediated release.

The microtubule stabilizer, deuterium oxide (D2O), at a concentration of 44% caused up to three-fold increase in release. This supports the belief that histamine release by this histamine-releasing factor (HRF) is a secretory process.

Indomethacin and 5,8,11,14-eicosatetraynoic acid (ETYA), which are modulators of arachidonic acid metabolism, produced little or no inhibition of histamine release by HRF, thus suggesting that the release is largely independent of the arachidonate system, probably unlike lgE-mediated release.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. D.O. Thueson, L.S. Speck, M.A. Lett-Brown andJ.A. Grant,Histamine-releasing activity (HRA): mononuclear cells, J. Immun.123, 626–632 (1979).

    PubMed  Google Scholar 

  2. J.S. Sedgwick, P.G. Holt andK.J. Turner,Production of a histamine-releasing lymphokine by antigen and mitogen-stimulated human peripheral T-cells, Clin. exp. Immunol.45, 399–408 (1981).

    PubMed  Google Scholar 

  3. I.C. Ezeamuzie andE.S.K. Assem.A study of histamine release from human basophils and lung mast cells by products of lymphocyte stimulation, Agents and Actions13, 222–230 (1983).

    PubMed  Google Scholar 

  4. D.C. Dumonde, R.A. Wolstencroft, G.S. Panayi.M. Matthew, J. Morley andW.T. Howson,Lymphokines: non-antibody mediators of cellular immunity generated by lymphocyte activation, Nature, Lond.224, 38–42 (1969).

    Google Scholar 

  5. R.A. Lewis, K.F. Austen, J.M. Drazen, A. Marfat andE.J. Corey,Slow-reacting substance of anaphylaxis: identification of leukotrienes C and D from human and rat sources, Proc. natn. Acad. Sci. USA77, 3710–3714 (1982).

    Google Scholar 

  6. I.C. Ezeamuzie andE.S.K. Assem,Histamine and SRS release from human leucocytes by lymphokines, Int. J. Immunopharm.4, 378 (1982).

    Article  Google Scholar 

  7. R.P. Siraganian, Histamine release and essay methods for the study of human allergy. InManual of Clinical Immunology, pp. 603–615 (EdsN.H. Rose andH. Friedman). American Society for Microbiology, Washington D.C. 1976.

    Google Scholar 

  8. E. Middleton Jr. andG. Drzewiecki,Effects of flavonoids and transitional metal cations on antigeninduced histamine release from human basophils, Biochem. Pharmac.31, 1449–1453 (1982).

    Article  Google Scholar 

  9. D. Marsland, L.G. Tilney andM. Hirshfield,Stabilizing effect of D 2 O on the microtubular components and needle-like form of heliozoam axopods —a pressure-temperature analysis, J. cell. comp. Physiol.77, 187–193 (1977).

    Google Scholar 

  10. E. Gillespie andL.M. Lichtenstein, Histamine release from human leucocytes: studies with deuterium oxide, colchicine and cytochalasin B, J. clin. Invest.51, 2941–2947 (1972).

    PubMed  Google Scholar 

  11. E. Gillespie, R.J. Levine andS.E. Malawista,Histamine release from rat peritoneal mast cells inhibited by colchicine and potentiation by deuterium oxide, J. Pharmac. exp. Ther.164, 158–165 (1968).

    Google Scholar 

  12. G. Marone, A. Kagey-Sobotka andL.M. Lichtenstein,Effect of arachidonic acid and its metabolites on antigen-induced histamine release from human basophils in vitro, J. Immun.123, 1669–1677 (1979).

    PubMed  Google Scholar 

  13. T.J. Sullivan andC.W. Parker,Possible role of arachidonic acid and its metabolites in mediator release from rat mast cells. J. Immun.122, 431–442 (1979).

    PubMed  Google Scholar 

  14. D.G. Ahern andD.T. Downing,Inhibition of prostaglandin biosynthesis by eicosa-5,8,11,14-tetraynoic acid, Biochim. biophys. Acta210, 456–461 (1970).

    PubMed  Google Scholar 

  15. E.A. Ham, V.J. Cirillo, M. Zanetti, T.Y. Shen andF.A. Kuehl, Studies on the mode of action of non-steroidal anti-inflammatory agents. InProstaglandins in Cellular Biology, pp. 345–352 (EdsP.W. Ramwell andB.B. Pharriss, Plenum Press. New York 1972.

    Google Scholar 

  16. T.C. Theoharides, W. Sieghart, P. Greengard andW.W. Doughlas,Antiallergic drug cromolyn may inhibit histamine secretion by regulating phosphorylation of a mast cell protein, Science207, 80–82 (1980).

    PubMed  Google Scholar 

  17. H. Glossmann, P. Presek andEigenbrodt,Quercetin inhibits tyrosine phosphorylation by the cyclic nucleotide-independent, transforming protein kinase pp60 Src, Naunyn-Schmeideberg's Arch. Pharmac.317, 100–102 (1981).

    Article  Google Scholar 

  18. R.E. Hughes andH.K. Wilson,Flavonoids: Some physiological and nutritional considerations, Prog. med. chem.14, 285–299 (1977).

    PubMed  Google Scholar 

  19. A.M. Poisner andJ. Bernstein,A possible role of microtubules in catecholamine release from the adrenal medulla: effect of colchicine vinca alkaloids and deuterium oxide. J. Pharmac. exp. Ther.177, 102–108 (1971).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology, University College London, Gower Street, WC1E 6BT, London

    I. C. Ezeamuzie & E. S. K. Assem

Authors
  1. I. C. Ezeamuzie
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. S. K. Assem
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by a grant from the University of Port Harcourt, Nigeria.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ezeamuzie, I.C., Assem, E.S.K. Modulation of the effect of histamine-releasing lymphokine on human basophils. Agents and Actions 14, 501–505 (1984). https://doi.org/10.1007/BF01973859

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01973859

Keywords

Search

Navigation