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Phospholipase A2 inhibitors modulate the effects of glutoxim and molixan on the intracellular Ca2+ level in macrophages

  • Biochemistry, Biophysics and Molecular Biology
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Abstract

Using the fluorescent Ca2+ probe Fura-2AM, the possible involvement of phospholipase A2, the key enzyme in the arachidonic acid cascade, in the effect of drugs glutoxim and molixan on the intracellular Ca2+ concentration in macrophages was studied. It was shown for the first time that preincubation of macrophages with the classical phospholipase A2 inhibitor, 4-bromophenacyl bromide, as well as with glucocorticosteroids prednisolone and dexamethasone significantly inhibits Ca2+ responses induced by glutoxim or molixan in macrophages. These results indicate the involvement of phospholipase A2 and arachidonic acid cascade in glutoximand molixan-induced signaling in macrophages.

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References

  1. Borisov, A.E., Kozhemyakin, L.A., Antushevich, A.E., et al., Vestn. Khirurgii im. I.I. Grekova, 2001, vol. 4, pp. 32–38.

    Google Scholar 

  2. Eremeev, V.V. and Gergert, V.Ya., Tuberkulez Bolezni Legkikh, 2013, vol. 7, pp. 43–47.

    Google Scholar 

  3. Kurilova, L.S., Krutetskaya, Z.I., Lebedev, O.E., et al., Cell Tissue Biol., 2008, vol. 2, pp. 322–332.

    Article  Google Scholar 

  4. Kurilova, L.S., Krutetskaya, Z.I., Lebedev, O.E., et al., Tsitologiya, 2011, vol. 53, no. 9, p. 708.

    Google Scholar 

  5. Krutetskaya, Z.I., Lebedev, O.E., and Kurilova, L.S., Mekhanizmy vnutrikletochnoi signalizatsii (Intracellular Signaling Mechanisms), St. Petersburg: Izd. St.-Petersb. Univ., 2003, 208 p.

    Google Scholar 

  6. Dennis, E.A., Amer. J. Respir. Crit. Care. Med., 2000, vol. 161, pp. 532–535.

    Google Scholar 

  7. Needleman, P., Turk, J., Jacksick, B.A., et al., Annu. Rev. Biochem., 1986, vol. 55, pp. 69–102.

    Article  PubMed  CAS  Google Scholar 

  8. Hafner, A.K., Cernescu, M., Hofmann, B., et al., J. Biol. Chem., 2011, vol. 392, pp. 1097–1111.

    Google Scholar 

  9. Krutetskaya, Z.I., Kurilova, L.S., Antonov, V.G., et al., Dokl. Biol. Sci., 2013, vol. 452, no. 6, pp. 2777–279.

    Google Scholar 

  10. Kurilova, L.S., Krutetskaya, Z.I., Naumova, A.A., et al., Tsitologiya, 2014, vol. 56, no. 5, pp. 353–360.

    CAS  Google Scholar 

  11. Kurilova, L.S., Krutetskaya, Z.I., Lebedev, O.E., et al., Tsitologiya, 2012, vol. 54, no. 2, pp. 135–142.

    CAS  Google Scholar 

  12. Grynkiewicz, G., Poenie, M., and Tsien, R.Y., J. Biol. Chem., 1985, vol. 260, pp. 3440–3450.

    PubMed  CAS  Google Scholar 

  13. Xie, Q., Zhang, Y., Zhai, C., et al., J. Biol. Chem., 2002, vol. 277, pp. 16559–16566.

    Article  PubMed  CAS  Google Scholar 

  14. Irvine, R.F., Biochem. J., 1982, vol. 204, pp. 3–16.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  15. Gewert, K. and Sundler, R., Biochem. J., 1995, vol. 307, pp. 499–504.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

Download references

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Authors and Affiliations

  1. St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia

    Z. I. Krutetskaya, L. S. Milenina, A. A. Naumova, V. G. Antonov & A. D. Nozdrachev

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  1. Z. I. Krutetskaya
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  2. L. S. Milenina
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  3. A. A. Naumova
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  4. V. G. Antonov
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  5. A. D. Nozdrachev
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Correspondence to Z. I. Krutetskaya.

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Original Russian Text © Z.I. Krutetskaya, L.S. Milenina, A.A. Naumova, V.G. Antonov, A.D. Nozdrachev, 2015, published in Doklady Akademii Nauk, 2015, Vol. 465, No. 2, pp. 249–251.

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Krutetskaya, Z.I., Milenina, L.S., Naumova, A.A. et al. Phospholipase A2 inhibitors modulate the effects of glutoxim and molixan on the intracellular Ca2+ level in macrophages. Dokl Biochem Biophys 465, 374–376 (2015). https://doi.org/10.1134/S1607672915060083

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  • DOI: https://doi.org/10.1134/S1607672915060083

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