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Selective and reversible modification of essential thiol groups of D-glyceraldehyde-3-phosphate dehydrogenase by isothiocyanates

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Summary

Aralkyl and alkyl isothiocyanates, like aryl isothiocyanates, undergo a selective and reversible reaction with essential thiol groups of D-glyceraldehyde-3-phosphate dehydrogenase; the former 2 substances require for a reversible reaction course a more alkaline medium and presence of a thiol.

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Literatur

  1. P. C. Jocelyn, Biochemistry of the SH Group Academic Press, New York 1972.

    Google Scholar 

  2. M. Friedman, The Chemistry and Biochemistry of the Sulfhydryl Group in Amino Acids, Peptides and Proteins, Pergamon Press, New York 1973.

    Google Scholar 

  3. A. N. Glazer, R. J. DeLange and D. S. Sigman, in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 4 part 1, p. 1. Ed. T. S. Work and E. Work. North-Holland Publishing Co, Amsterdam 1976.

    Google Scholar 

  4. W. B. Jakoby and M. Wilchek, in: Methods in Enzymology, vol. 46. Academic Press, New York 1977.

    Google Scholar 

  5. Ľ. Drobnica and P. Gemeiner, colln. Czech. chem. Commun.40, 3346 (1975).

    Article  CAS  Google Scholar 

  6. Ľ. Drobnica and P. Gemeiner, in: Protein Structure and Evolution, p. 105. Ed. J. L. Fox, Z. Deyl and A. Blažej Marcel Dekker, New York 1976.

    Google Scholar 

  7. Ľ. Drobnica, P. Kristián and J. Augusín, in: The Chemistry of Cyanate and their Thio Derivatives, part 2, p. 1003. Ed. S. Patai, J. Wiley and Sons, Chichester 1977.

    Google Scholar 

  8. J. I. Harris and M. Waters, in: The Enzymes, 3rd edn, vol. 13, part C, p. 1 Ed. P. D. Boyer, Academic Press, New York 1976.

    Google Scholar 

  9. Ch. S. Furfine and S. F. Velick, J. biol. Chem.240, 844 (1965).

    Article  CAS  PubMed  Google Scholar 

  10. R. Jaenicke, D. Schmid and S. Knof, Biochemistry7, 926 (1968).

    Article  Google Scholar 

  11. Ľ. Drobnica, D. Podhradský and P. Gemeiner, Colln. Czech. chem. Commun.40, 3688 (1975).

    Article  CAS  Google Scholar 

  12. J. Augustín, Ľ. Drobnica and F. Kouřil, Chem. Zvesti20, 687 (1965).

    Google Scholar 

  13. R. A. MacQuarrie and S. A. Bernhard, Biochemistry10, 2456 (1971).

    Article  CAS  PubMed  Google Scholar 

  14. A. Levitzki, Biochem. biophys. Res. Commun.54, 889 (1973).

    Article  CAS  PubMed  Google Scholar 

  15. A. Levitzki, J. molec. Biol.90, 451 (1974).

    Article  CAS  PubMed  Google Scholar 

  16. D. J. Smith, E. T. Maggio and G. L. Kenyon, Biochemistry14, 766 (1975).

    Article  CAS  PubMed  Google Scholar 

  17. P. Gemeiner and Ľ. Drobnica, Colln. Commun. Slovak Inst. Technol. 1975–1976; p. 331. Alfa, Bratislava 1978.

  18. P. Gemeiner, J. Augusín and Ľ. Drobnica, Carbohyd. Res.53, 217 (1977).

    Article  CAS  Google Scholar 

  19. P. Gemeiner, Ľ. Drobnica and K. Poláková, J. solid-phase Biochem.2, 289 (1977).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta, 809 33, Bratislava, Czechoslovakia

    P. Gemeiner & Ľ. Drobnica

  2. Department of Microbiology and Biochemistry, Slovak Institute of Technology, 880 37, Bratislava, Czechoslovakia

    P. Gemeiner & Ľ. Drobnica

Authors
  1. P. Gemeiner
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  2. Ľ. Drobnica
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Additional information

The authors are indebted to Dr J. Augustín for valuable discussions, to Dr E. Režná and Dr M. Gemeinerová for their technical assistence. The authors wish also to thank Dr H. R. Kricheldorf for sample of 4-(trimethylsilyl) butanoyl isothiocyanate

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Gemeiner, P., Drobnica, Ľ. Selective and reversible modification of essential thiol groups of D-glyceraldehyde-3-phosphate dehydrogenase by isothiocyanates. Experientia 35, 857–859 (1979). https://doi.org/10.1007/BF01955110

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  • DOI: https://doi.org/10.1007/BF01955110

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