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Calcium release from frog sarcoplasmic reticulum by an imidazolyl reagent

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Summary

Calcium is released from the isolated heavy sarcoplasmic reticulum (SR) of frog skeletal muscle upon application of 0.1–1 mM diethylpyrocarbonate (DEP, an imidazolyl reagent). The Ca-ATPase activity of SR was suppressed by 20% in the presence of 1 mM DEP. More than 1 mM of free magnesium ion or 5 μM ruthenium red eliminated the effect of DEP on calcium release but not on Ca-ATPase activity. A plausible site of DEP action is on the calcium channel.

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Authors and Affiliations

  1. Department of Physiology, Nagoya City University Medical School, Mizuho-ku, 467, Nagoya, Japan

    T. Oba

  2. Department of Food and Nutrition Science, Gifu City Women's Junior College, 2693 Nagarafukumitsu, 502, Gifu, Japan

    T. Aoki

Authors
  1. T. Aoki
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  2. T. Oba
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Aoki, T., Oba, T. Calcium release from frog sarcoplasmic reticulum by an imidazolyl reagent. Experientia 45, 987–991 (1989). https://doi.org/10.1007/BF01953058

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  • DOI: https://doi.org/10.1007/BF01953058

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