Summary
Mesophyll protoplasts ofHyoscyamus niger var.annuus could be isolated enzymatically. They showed cell division and developed into cell clusters.
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References
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O. L. Gamborg andL. R. Wetter,Plant Tissue Culture Methods (published by the National Research Council of Canada 1975).
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Supported by a grant of the Bundesministerium für Forschung und Technologie, Bonn.
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Kohlenbach, H.W., Bohnke, E. Isolation and culture of mesophyll protoplasts ofHyoscyamus niger L. var.annuus sims. Experientia 31, 1281–1283 (1975). https://doi.org/10.1007/BF01945781
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DOI: https://doi.org/10.1007/BF01945781