Summary
Suspension cultures ofVicia hajastana andHaplopappus gracilis were maintained in B5 medium containing 0.1, 1.0 and 10.0, and 0.1, 0.5, 1.0 and 5.0 μg/ml 2,4-D, respectively. Anaphase analyses showed that the frequency of anomalies, especially bridges, was negatively associated with the 2,4-D concentration.
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The present study was conducted at Crop Science Department, University of Saskatchewan, Saskatoon, Canada. I am thankful to Dr.C. Lehman, Gatersleben, and Dr.T. Eriksson, Uppsala, for the gift ofVicia seeds andHaplopappus cultures, respectively.
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Singh, B.D., Harvey, B.L. Does 2,4-D induce mitotic irregularities in plant tissue cultures?. Experientia 31, 785–787 (1975). https://doi.org/10.1007/BF01938465
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DOI: https://doi.org/10.1007/BF01938465