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Nitric oxide-dependent blood-brain barrier permeability alteration in the rat brain

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Abstract

The role of nitric oxide (NO), a well known vasodilator, in the regulation of blood-brain barrier (BBB) permeability is not clear. Therefore, the present study was planned to assess the role of NO-releasing compounds like sodium nitroprusside (SNP) and the active metabolite of molsidomine, SIN-1, as well as a precursor of NO, L-arginine, on this physiological barrier. The permeability was assessed by using several tracers. All three agents increased the permeability of BBB to the tracer. The increase in permeability caused by L-arginine was not blocked by N-nitro-L-arginine methyl ester (L-NAME). L-Arginine-treated brains did not show an elevation of nitrite content, thus ruling out the possibility of NO generation and its involvement in BBB permeability alteration. It is concluded that NO itself causes an increase in the permeability of BBB. However arginine-induced opening is not NO mediated.

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Authors and Affiliations

  1. Pharmacology Division, Central Drug Research Institute, 226001, Lucknow, India

    A. Shukla & M. Dikshit

  2. Industrial Toxicology Research Centre, 226001, Lucknow, India

    R. C. Srimal

Authors
  1. A. Shukla
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  2. M. Dikshit
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  3. R. C. Srimal
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CDRI Communication Number: 5178.

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Shukla, A., Dikshit, M. & Srimal, R.C. Nitric oxide-dependent blood-brain barrier permeability alteration in the rat brain. Experientia 52, 136–140 (1996). https://doi.org/10.1007/BF01923358

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  • DOI: https://doi.org/10.1007/BF01923358

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