Summary
The effects of Mn++, La+++ and Co++ (2 mM) with and without ISO or DBcAMP were investigated in isolated perfused rat hearts concerning mechanical and metabolic parameters. Mn++ and La+++ produce cardiac arrest accompanied by unchanged content of high energy phosphates. ISO or DBcAMP resp.administered together with these cations do not exhibit characteristic effects. Co++ induces cardiac arrest as well, but in addition severe reduction of high energy phosphate stores and O2-consumption. The effects of Co++ can be partly prevented by administration of ISO and elevated extracellular Ca++ concentration concerning mechanical parameters and oxygen consumption, whereas high energy phosphate stores remain low. The results obtained demonstrate that the Ca++ antagonstic ions studied show the expected metabolic consequences of EC-uncoupling only in the case of Mn++ and La+++. Co++ ions induce in additions to cardiac arrest and low O2-consumption severe reduction of high energy phosphate stores indicating disturbance of respiratory control of mitochondria.
Zusammenfassung
Die Wirkungen von Mn++, La+++ und Co++ (2 mM) wurden an isoliert perfundierten Rattenherzen jeweils mit und ohne gleichzeitige Applikation von Isoproterenol (ISO) oder DBcAMP bezüglich mechanischer und metabolischer Parameter untersucht.
Mn++ und La+++ verursachen einen Herzstillstand, der von einem unveränderten Gehalt an energiereichen Phosphaten begleitet ist. Die Gabe von ISO oder DBcAMP zusammen mit den genannten Kationen hat keine weiteren Veränderungen zur Folge. Co++ verursacht ebenfalls einen Herzstillstand, aber außerdem eine ausgeprägte Verminderung der Vorräte an energiereichem Phosphat und des O2-Verbrauchs. Die Co++-Effekte können durch ISO und erhöhte Ca++-Konzentrationen teilweise verhindert werden, während die Vorräte an energiereichem Phosphat niedrig bleiben. Die Versuche zeigen, daß Ca++-antagonistische Ionen die erwarteten metabolischen Folgen einer elektromechanischen Entkoppelung nur im Falle von La+++ und Mn++ zeigen. Co-Ionen erzeugen zusätzlich zum Herzstillstand und zu niedrigem O2-Verbrauch eine ausgeprägte Reduktion der Vorräte an energiereichem Phosphat, die auf eine Störung der mitochondrialen Atmungskontrolle hinweist.
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With 3 figures and 1 table
The results were partly published in Pflügers Arch. 355, R6, 1975, and Pflügers Arch.373, R26, 1978
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Giesen, J., Kammermeier, H. Myocardial high energy phosphates and function as influenced by di- or trivalent cations and isoproterenol or DBcAMP. Basic Res Cardiol 76, 152–162 (1981). https://doi.org/10.1007/BF01907954
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DOI: https://doi.org/10.1007/BF01907954