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Evidence against H+−HCO −3 symport as the mechanism for HCO −3 transport in the cyanobacteriumAnabaena variabilis

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Summary

The rate of inorganic carbon uptake and its steadystate accumulation ratio (intracellular/extracellular concentration) was determined in the cyanobacteriumAnabaena variabilis as a function of extracellular pH. The free energy of protons (\(\Delta \overline \mu _{H^ - }\)) across the plasmalemma was calculated from determinations of membrane potential, and intracellular pH, as a function of the extracellular pH. While inward proton motive force decreased with increasing extracellular pH from 6.5 to 9.5, rate of HCO −3 influx and its accumulation ration increased. The latter is several times larger than would be expected should HCO −3 influx be driven by\(\Delta \overline \mu _{H^ + }\). It is concluded that HCO −3 transport in cyanobacteria is not driven by the proton motive force.

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Authors and Affiliations

  1. Department of Botany, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel

    Drora Zenvirth, Micha Volokita & Aaron Kaplan

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  1. Drora Zenvirth
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  2. Micha Volokita
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  3. Aaron Kaplan
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Zenvirth, D., Volokita, M. & Kaplan, A. Evidence against H+−HCO −3 symport as the mechanism for HCO −3 transport in the cyanobacteriumAnabaena variabilis . J. Membrain Biol. 79, 271–274 (1984). https://doi.org/10.1007/BF01871065

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  • DOI: https://doi.org/10.1007/BF01871065

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