Summary
A study has been conducted to determine the ionic and metabolic requirements for full expression of the hydroosmotic response to antidiuretic hormone in the toad urinary bladder. By appropriate manipulation of incubation conditions it can be shown that there is a pool of serosal sodium necessary for a full hormone response. This serosal sodium pool is not related to the transepithelial sodium transport pool. A full hydroosmotic response also requires serosal potassium; however, no specific anion requirement was demonstrated. Additionally, anaerobic or aerobic metabolism support a full hydroosmotic response equally well.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bentley, P.J. 1958. The effects of neurohypophyseal extracts on water transfer across the wall of the isolated urinary bladder of the toadBufo marinus.J. Endocrinol. 17:201
Bentley, P.J. 1959. The effects of ionic changes on water transfer across the isolated urinary bladder of the toadBufo marinus.Endocrinology 18:327
Davis, W.L., Goodman, D.B.P., Schuster, R.J., Rasmussen, H., Martin, J.H. 1974. Effects of cytochalasin B on the response of toad urinary bladder to vasopressin.J. Cell Biol. 63:986
Finn, A.L., Handler, J.S., Orloff, J. 1966. Relation between toad bladder potassium content and permeability response to vasopressin.Am. J. Physiol. 210:1279
Goodman, D.B.P., Allen, J.E., Rasmussen, H. 1969. On the mechanism of action of aldosterone.Proc. Nat. Acad. Sci. USA 67:305
Handler, J.S., Petersen, M., Orloff, J. 1966. Effect of metabolic inhibitors on the response of the toad bladder to vasopressin.Am. J. Physiol. 211:1175
Handler, J.S., Preston, A.S., Orloff, J. 1969. The effect of aldosterone on glycolysis in the urinary bladder of the toad.J. Biol. Chem. 244:3194
Harris, I., Meriwether, B.P., Park, J.H. 1963. Chemical nature of the catalytic sites in glyceraldehyde-3-phosphate dehydrogenase.Nature (London) 198:154
Leaf, A. 1965. Transepithelial transport and its hormonal control in toad bladder.Ergeb. Physiol. 56:216
Ling, G.L. 1962. Physical theory of the living state: The association-induction hypothesis, with consideration of the mechanisms involved in ionic specificity. (Appendix H.) Blaisdell, New York
Maren, T.H. 1967. Carbonic anhydrase: Chemistry, physiology and inhibition.Physiol. Rev. 47:595
Parisi, M., Piccinni, Z.F. 1973. The penetration of water into the epithelium of toad urinary bladder and its modification by oxytocin.J. Membrane Biol. 12:227
Rasmussen, H., Schwartz, I.L., Schoessler, M.A., Hochster, G. 1960. Studies on the mechanism of vasopressin.Proc. Nat. Acad. Sci. USA 46:1278
Schwartz, I.L., Rasmussen, H., Schoessler, M.A., Silver, L., Fong, C.T.O. 1960. Relation of chemical attachment to physiological action of vasopressin.Proc. Nat. Acad. Sci. USA 46:1288
Skou, J.C. 1965. Enzymatic basis for active transport of sodium and potassium across cell membranes.Physiol. Rev. 45:596
Slater, E.C. 1967. Application of inhibitors and uncouplers for a study of oxidative phosphorylation.Methods Enzymol. 10:48
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Davis, W.L., Goodman, D.B.P. & Rasmussen, H. Ionic and metabolic requirements for the hydroosmotic response to antidiuretic hormone in toad urinary bladder. J. Membrain Biol. 41, 225–234 (1978). https://doi.org/10.1007/BF01870430
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01870430