Summary
The ability of early proximal tubule cells of theNecturus kidney to regulate volume was evaluated using light microscopy, video analysis and conventional microelectrodes.Necturus proximal tubule cells regulate volume in both hyperand hyposmotic solutions. Volume regulation in hyperosmotic fluids is HCO −3 dependent and is associated with a decrease in the relative K+ conductance of the basolateral cell membrane and a decrease in the resistance ratio,R a /R bl . Volume regulation in hyposmotic solutions is also dependent upon the presence of HCO −3 but is also inhibited by 2mm Ba2+ in the basolateral solution. Hyposmotic regulation is accompanied by an increase in the relative K+ conductance of the basolateral cell membrane and an increase inR a /R bl . Neither hypo- nor hyposmotic regulation have any affect on the depolarization of the basolateral cell membrane potential induced by HCO −3 removal. We conclude that volume regulation in the early proximal tubule of the kidney involves both HCO −3 -dependent transport systems and the basolateral K+ conductance.
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References
Boulpaep, E.L. 1976. Electrical phenomena in the nephron.Kidney Int. 9:88–102
Burg, M., Grantham, J., Abramow, M., Orloff, J. 1966. Preparation and study of fragments of single rabbit nephrons.Am. J. Physiol. 210:1293–1298
Cala, P.M. 1980. Volume regulation byAmphiuma red blood cells: The membrane potential and its implication regarding the nature of the ion-flux pathways.J. Gen. Physiol. 76:683–708
Dellasega, M., Grantham, J.J. 1973. Regulation of renal tubule cell volume in hypotonic media.Am. J. Physiol. 244:1288–1294
Ericson, A.-C., Spring, K.R. 1982. Volume regulation byNecturus gallbladder: Apical Na+−H+ and Cl−HCO −3 exchange.Am. J. Physiol. 243:C146-C150
Fisher, R.S., Persson, B.-E., Spring, K.R. 1981. Epithelial cell volume regulation: Bicarbonate dependence.Science 214:1357–1359
Fisher, R.S., Spring, K.R. 1984. Intracellular activities during volume regulation byNecturus gallbladder.J. Membrane Biol. 78:187–199
Gagnon, J., Ouimet, D., Nguyen, H., Laprade, R., Le Grimellec, C., Carriere, S., Cardinal, J. 1982. Cell volume regulation in the proximal convoluted tubuleAm. J. Physiol. 243:F408-F415
Giebisch, G., Sullivan, L.P., Whittembury, G. 1973. Relationship between net sodium reabsorption and peritubular potassium uptake in the perfusedNecturus kidney.J. Physiol. (London) 230:51–74
Grasset, E., Gunter-Smith, P., Schultz, S.G. 1983. Effects of Na-coupled alanine transport on intracellular K activities and the K conductance of the basolateral membranes ofNecturus small intestine.J. Membrane Biol. 71:89–94
Guggino, W.B. 1986. Functional heterogeneity in the early distal tubule of theAmphiuma kidney: Evidence for two modes of Cl− and K+ transport across the basolateral cell membrane.Am. J. Physiol. 250:F430-F440
Guggino, W.B., Boulpaep, E.L., Giebisch, G. 1982. Electrical properties of chloride transport across theNecturus proximal tubule.J. Membrane Biol. 65:185–196
Guggino, W.B., London, R., Boulpaep, E.L., Giebisch, G. 1983. Chloride transport across the basolateral cell membrane of theNecturus proximal tubule: Dependence on bicarbonate and sodium.J. Membrane Biol. 71:227–240
Guggino, W.B., Oberleithner, H., Giebisch, G. (Appendices by L.M. Amzel) 1985. Relationship between cell volume and ion transport in the early distal tubule of theAmphiuma kidney.J. Gen. Physiol. 86:31–58
Hebert, S.C. 1986a. Hypertonic cell volume regulation in mouse thick limbs: I. ADH dependency and nephron heterogeneity.Am. J. Physiol. 250:C907-C919
Hebert, S.C. 1986b. Hypertonic cell volume regulation in mouse thick limbs: II. Na+−H+ and Cl−−HCO 3− exchange in basolateral membranes.Am. J. Physiol. 250:C920-C931
Kregenow, F.M. 1981. Osmoregulatory salt transporting mechanisms: Control of cell volume in anisotonic media.Annu. Rev. Physiol. 43:493–505
Kregenow, J.M., Caryk, T., Siebens, A.W. 1985. Further studies of the volume-regulatory response ofAmphiuma red cells in hypertonic media: Evidence for amiloride-sensitive Na/H exchange.J. Gen. Physiol. 86:565–584
Larson, M., Spring, K.R. 1983. KCl cotransport during epithelial cell volume regulation.Fed. Proc. 42:987
Lau, K.R., Hudson, R.L., Schultz, S.G. 1984. Cell swelling increases a barium-sensitive potassium conductance in the basolateral cell membrane ofNecturus small intestine.Proc. Natl. Acad. Sci. USA 81:3591–3594
Lau, K.R., Hudson, R.L., Schultz, S.G. 1986. Effect of hypertonicity on the increase basolateral conductance ofNecturus small intestine in response to Na+-sugar cotransport.Biochim. Biophys. Acta 855:193–196
Law, R. 1985. Volume regulation by mammalian renal cells exposed to anisotonic media.Mol. Physiol. 8:143–160
London, R., Cohen, B., Guggino, W.B., Giebisch, G. 1983. Regulation of intracellular chloride activity during perfusion with hypertotic solutions in theNecturus proximal tubule.J. Membrane Biol. 75:253–258
Lopes, A.G., Siebens, A., Giebisch, G., Boron, W. 1987. Electrogenic Na−HCO3 cotransport across the basolateral membrane of isolated and perfusedNecturus proximal tubule.Am. J. Physiol. (in press)
Matsumura, Y., Cohen, B., Guggino, W.B., Giebisch, G. 1984a. Electrical effects of potassium and bicarbonate on proximal tubule cells ofNecturus.J. Membrane Biol. 79:145–152
Matsumura, Y., Cohen, B., Guggino, W.B., Giebisch, G. 1984b. Regulation of basolateral potassium conductance of theNecturus proximal tubule.J. Membrane Biol. 79:153–161
Messner, G., Oberleithner, H., Lang, F. 1985. The effect of phenylalanine on the electrical properties of proximal tubule cells in the frog kidney.Pfluegers Arch. 404:138–144
Reuss, L., Finn, A.L. 1977. Effects of luminal hyperosmolality on electrical pathways ofNecturus gallbladder.Am. J. Physiol. 232:C99-C108
Richards, N.W., Dawson, D.C. 1986. Single potassium channels blocked by lidocaine and quinidine in isolated turtle colon epithelial cells.Am. J. Physiol. 251:C85-C89
Shindo, T., Spring, K.R. 1981. Chloride movement across the basolateral membrane of proximal tubule cells.J. Membrane Biol. 58:35–42
Siebens, A.W., Kregenow, F.M. 1985. Volume-regulatory responses ofAmphiuma red cells in anisotonic media: Effect of amiloride.J. Gen. Physiol. 86:527–564
Spring, K.R., Giebisch, G. 1977. Kinetics of Na+ transport inNecturus proximal tubule.J. Gen. Physiol. 70:307–328
Spring, K.S., Kimura, G. 1978. Chloride reabsorption by renal proximal tubules ofNecturus.J. Membrane Biol. 38:233–254
Ussing, H.H. 1965. Relationship between osmotic reactions and active sodium transport in the frog skin epithelium.Acta Physiol. Scand. 63:141–155
Welling, P.A., Linshaw, M.A., Sullivan, L.P. 1985. Effect of barium on cell volume regulation in rabbit proximal straight tubules.Am. J. Physiol. 249:F20-F27
Whittembury, G., Hill, B.S. 1982. Fluid reabsorption byNecturus proximal tubule perfused with solutions of normal and reduced osmolarity.Proc. R. Soc. London B. 215:411–434
Zeiske, W., Van Driessche, W. 1984. The sensitivity of apical Na+ permeability in frog skin to hypertonic stress.Pfluegers Arch. 400:130–139
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Lopes, A.G., Guggino, W.B. Volume regulation in the early proximal tubule of theNecturus kidney. J. Membrain Biol. 97, 117–125 (1987). https://doi.org/10.1007/BF01869418
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DOI: https://doi.org/10.1007/BF01869418