Summary
Different groups of NMRI-mice were given single doses of 2, 10, 50, 250 or 1000 mg/kg of Cph or 6-MP. Other groups were applicated 7×10 mg/kg every alternate day. 2 days after the last dose lymph node and spleen cells were collected and stimulated with PHA. Stimulation was estimated by the quotient of the uptake of3H-thymidine in stimulated and unstimulated cultures. Controls were untreated animals. It could be demonstrated that only maximal doses could inhibit the stimulation. Cph was more efficient than 6-MP. Lymph node cells were more sensitive than spleen cells. Repeated application showed no effect.
The results demonstrate that cytostatic drugs can inhibit blast cell transformationin vivo. The different results are due to the different mode of action of those substances and from the different populations of harvested cells.
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We thank Dipl.-Phys. R. Neuwirth for the statistical analysis.
Cyclophosphamid (Cph) = Endoxan®. 6-mercaptopurin (6-MP) = Puri-nethol. Phythämagglutinin (PHA) Borroughs, Wellcome & Co.
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Scheiffarth, F., Leykam, D., Warnatz, H. et al. Effect of cytostatic drugs on blast cell transformation of lymph node and spleen cells. Res. Exp. Med. 160, 196–205 (1973). https://doi.org/10.1007/BF01856783
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DOI: https://doi.org/10.1007/BF01856783