We studied the chronodependent effect of IL-2 in the experimental model of immunodeficiency, cyclophosphamide-induced immunosuppression in mice. IL-2 in a dose of 100 U/ mouse was administered at 10.00 and 16.00 for 3 days after injection of cyclophosphamide. In contrast to the morning treatment with the cytokine, evening administration produced antiapoptotic effect on splenocytes and stimulated proliferation to a greater extent. This was accompanied by an increase in the number of CD4+, CD25+ and CD4+25+ cells in the spleen to a level of intact mice. More pronounced effect of the evening mode of IL-2 administration on the proliferation and subpopulation composition of mouse spleen cells in the studied model can be associated with high blood level of CD25+ cells at this time of the day.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 158, No. 10, pp. 472-475, October, 2014
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Shurlygina, A.V., Mel’nikova, E.V. & Trufakin, V.A. Chronodependent Effect of Interleukin-2 on Mouse Spleen Cells in the Model of Cyclophosphamide Immunosuppression. Bull Exp Biol Med 158, 471–474 (2015). https://doi.org/10.1007/s10517-015-2787-y
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DOI: https://doi.org/10.1007/s10517-015-2787-y