Skip to main content
SpringerLink
Log in

Intrazelluläres freies Kalzium und Plasma-Kalzium bei Patienten mit essentieller Hypertonie

Intracellular free calcium and serum-calcium in essential hypertension

Besteht ein Zusammenhang zwischen extrazellulären und intrazellulären Störungen der Kalziumregulation bei essentieller Hypertonie?

  • Originalien
  • Published:
Klinische Wochenschrift Aims and scope Submit manuscript

Summary

Alterations in intracellular free calcium regulation as well as disturbances of extracellular calcium homeostasis have been observed in patients with essential hypertension. It has been hypothesized that a generalized defect of calcium regulation might be of importance in the pathogenesis of essential hypertension. In one hypothesis a primary calcium-deficiency in essential hypertension has been linked to subsequent membrane instability and altered intracellular free calcium concentrations.

We therefore investigated the relationship between intracellular free calcium concentrations in platelets and ionized as well as total plasma calcium in patients (n=38) with essential hypertension and in age-matched normotensive subjects (n=35). Intracellular free calcium in hypertensive patients was elevated by 13% (p<0.05) although there was a wide overlap between the values of the two groups. However, no difference with regard to the ionized or total plasma calcium concentrations could be observed. Total plasma calcium concentration, ionized plasma levels as well as intracellular free calcium were positively correlated (p<0.05) with mean arterial blood pressure. No correlation however was found between the extracellular and intracellular calcium compartments.

We therefore could not observe a generalized defect of calcium regulation in essential hypertension, and extracellular calcium concentrations do not seem to be related to intracellular calcium derangement in this disease. Furthermore, we can conclude from our data that there is no evidence for the so-called calcium deficiency hypothesis in patients with essential hypertension.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Aalkjaer C, Heagerty AM, Parvin SD, Bell PR, Bing RF, Swales JD (1986) Cell membrane sodium transport: a correlation between human resistance vessels and leucocytes. Lancet 4:649

    Google Scholar 

  2. Aksoy MO, Mras S, Kamm KE, Murphy RA (1983) Ca2+, cAMP, and changes in myosin phosphorylation during contraction of smooth muscle. Am J Physiol 245:C255

    Google Scholar 

  3. Blaustein MP (1977) Sodium ions, calcium ions, blood pressure regulation and hypertension: a reassessment and a hypothesis. Am J Physiol 232:C165

    Google Scholar 

  4. Bradley AB, Morgan KG (1985) Cellular Ca2+ monitored by aequorin in adenosine-mediated smooth muscle relaxation. Am J Physiol 248:H109

    Google Scholar 

  5. Brodde OE, Kretsch R, Ikezono K, Zerkowski HR, Reidemeister JC (1986) Human β-adrenoceptors; relation of myocardial and lymphocyte β-adrenoceptor density. Science 231:1584

    Google Scholar 

  6. Bruschi G, Bruschi ME, Caroppo M, Orlandini G, Spaggiari M, Cavatorta A (1985) Cytoplasmic free [Ca2+] is increased in the platelets of spontaneously hypertensive rats and essential hypertensive patients. Clin Sci 68:179

    Google Scholar 

  7. Bruschi G, Bruschi ME, Caroppo M, Orlandini G, Pavarani C, Cavatorta A (1984) Intracellular free [Ca2+] in circulating lymphocytes of spontaneously hypertensive rats. Life Sci 35:535

    Google Scholar 

  8. Bulpitt CJ, Hodes C, Everitt MG (1976) The relationship between blood pressure and biochemical risk factors in a general population. Br J Prevent Soc Med 30:158

    Google Scholar 

  9. Cooper RS, Shamsi N, Katz S (1987) Intracellular calcium and sodium in hypertensive patients. Hypertension 9:224

    Google Scholar 

  10. Doyle AE, Fraser JRE, Marshall RJ (1959) Reactivity of forearm vessels to vasoconstrictor substances in hypertensive and normotensive subjects. Clin Sci 18:441

    Google Scholar 

  11. Erne P, Bolli P, Burgisser E, Bühler FR (1984) Correlation of platelet calcium with blood pressure. N Engl J Med 310(17):1084

    Google Scholar 

  12. Favre L, Lew PD, Valloton MB (1984) Cytosolic free calcium and intracellular calcium stores are normal in neutrophils from patients with essential hypertension. J Hypertension 2 (suppl 3):493

    Google Scholar 

  13. Folsom AR, Smith CL, Prineas RJ, Grimm RH Jr (1986) Serum calcium fractions in essential hypertensive and matched normotensive subjects. Hypertension 8:11

    Google Scholar 

  14. Haller H, Lenz T, Lüdersdorf M, Distler A, Philipp Th (1987) Intracellular free calcium in platelets and hypertension. J Clin Hypertension 3 (1):12

    Google Scholar 

  15. Haller H, Lenz T, Lüdersdorf M, Distler A, Philipp Th (1985) Changes in intracellular free platelet calcium during mineralocorticoid-induced blood pressure increase. J Hypertension 3 (suppl 3):48

    Google Scholar 

  16. Hermsmeyer K (1984) Altered arterial muscle ion transport mechanism in the spontaneously hypertensive rat. J Cardiovasc Pharmacol 6:S10

    Google Scholar 

  17. Hinssen H, D'Haese J, Small JV, Sobieszek A (1978) Mode of filament assembly of myosins from muscle and nonmuscle cells. J Ultrastruct Res 64:282

    Google Scholar 

  18. Jones AW (1973) Altered ion transport in vascular smooth muscle from spontaneously hypertensive rats, influences of aldosterone, norepinephrine and angiotensin. Circ Res 33:563

    Google Scholar 

  19. Kesteloot H, Geboers J (1983) Calcium and blood pressure. Lancet 1:813

    Google Scholar 

  20. Kesteloot H (1984) Epidemiological studies on the relationship between sodium, potassium, calcium, and magnesium and arterial blood pressure. J Cardiovasc Pharmacol 6 (suppl 1):S192

    Google Scholar 

  21. Klaus D, Antoni D, Lederle RM, Lemke R (1984) Ionisiertes Kalzium im Blut bei essentieller Hypertonie. Nieren- und Hochdruckkrankheiten 13:324

    Google Scholar 

  22. Kuriyama H, Ito Y, Suzuki H, Kitamura K, Itoh T (1982) Factors modifying contraction-relaxation cycle in vascular smooth muscles. Am J Physiol 243:H641

    Google Scholar 

  23. Le Quan Sang KH, Devynck M (1986) Increased platelet cytosolic free calcium concentration in essential hypertension. Hypertension 4:567

    Google Scholar 

  24. Lechi A, Lechi C, Bonadonna G, Sinigaglia D, Corradini P, Polignano R, Arosio E, Covi G, DeTogni P (1987) Increased basal and thrombin-induced free calcium in platelets of essential hypertensive patients. Hypertension 9:230

    Google Scholar 

  25. Lenz T, Haller H, Lüdersdorf M, Kribben A, Thiede HM, Distler A, Philipp Th (1985) Free intracellular calcium in essential hypertension effects of nifedipine and captopril. J Hypertension 3 (suppl 3):S13

    Google Scholar 

  26. Lindner A, Kenny M, Meachaus AJ (1987) Effects of a circulating factor in patients with essential hypertension on intracellular free calcium in normal platelets. NEJM 316, 9:509

    Google Scholar 

  27. McCarron DA, Pingree PA, Rubin RJ, Gaucher SM, Molitch M, Krutzik S (1986) Enhanced parathyroid function in essential hypertension: a homeostatic response to a urinary calcium leak. Hypertension 2:162

    Google Scholar 

  28. McCarron DA, Yung NN, Ugoretz BA, Krutzik S (1981) Disturbances of calcium metabolism in the spontaneously hypertensive rat. Hypertension 3 (suppl I):I-152

  29. McCarron DA (1985) Is calcium more important than sodium in the pathogenesis of essential hypertension? Hypertension 7:607

    Google Scholar 

  30. McCarron DA (1982) Low serum concentrations of ionized calcium in patients with hypertension. N Engl J Med 307:226

    Google Scholar 

  31. McLean AJ, duSouich P, Barron KW, Briggs AH (1978) Interaction of hydrazaline with tension development and mechanisms of calcium accumulation oin K+-stimulated rabbit aortic strips. J Pharmacol Exp Ther 207:40

    Google Scholar 

  32. Mendlowitz M, Naftchi NE, Wolf RL, Gitlow SE (1965) Vascular responsiveness in hypertensive and hypotensive stages. Geriatrics 20:797

    Google Scholar 

  33. Philipp Th, Distler A, Cordes U (1978) Sympathetic nervous system and blood pressure control in essential hypertension. Lancet 4:959

    Google Scholar 

  34. Postnov YV, Orlov SN (1984) Cell membrane alteration as a source of primary hypertension. J Hypertension 2:1

    Google Scholar 

  35. Postnov YV (1981) Alteration of cell membrane control over intracellular calcium in essential hypertension and in spontaneously hypertensive rats. In: Laragh JH, Buckler FR, Seldin DW (eds) Frontiers in hypertension research. Springer, New York, p 91

    Google Scholar 

  36. Postnov YV, Orlov SN (1980) Evidence of altered calcium accumulation and calcium binding by the membranes of adipocyts in spontaneously hypertensive rats. Pfluegers Arch 385:85

    Google Scholar 

  37. Rasmussen H (1983) Pathways of amplitude and sensitivity modulation in the calcium messenger system. In: Cheung WY (ed) Calcium and Cell Function, Vol. IV. Academic Press, New York, pp 1–63

    Google Scholar 

  38. Resnick LM, Laragh JH, Sealey JE, Aldermann MH (1983) Divalent cations in essential hypertension: relations between serum ionized calcium, magnesium and plasma renin activity. N Engl J Med 309(15):888

    Google Scholar 

  39. Rink TJ, Smoth SW, Tsien RY (1982) Cytoplasmic free Ca2+ in human platelets: Ca2+ thresholds and Ca-independent, activation for shape-change and aggregation. FEBS letters 148:21

    Google Scholar 

  40. Robinson BF, Dobbs RJ, Bayley S (1982) Response of forearm resistance vessels to verapamil and sodium nitroprusside in normotensive and hypertensive men: evidence for a functional abnormality of vascular smooth muscle in primary hypertension. Clin Sci 63:33

    Google Scholar 

  41. Robinson BF (1984) Altered calcium handling as a cause of primary hypertension. J Hypertension 2:453

    Google Scholar 

  42. Robinson D, Bailey AR, Williams PT (1982) Calcium and blood pressure. Lancet 2:1215

    Google Scholar 

  43. Shore AC, Beynon GW, Jones JC, Markandu ND, Sagnella GA, MacGregor GA (1985) Mononuclear leucocyte intracellular free calcium — does it correlate with blood pressure? J Hypertension 3:183

    Google Scholar 

  44. Stern N, Lee DBN, Beck PWJ, Sills V, Deftos L, Sowers JR (1983) High calcium diet reverses abnormal calcium and PTH concentrations but does not prevent hypertension in young spontaneously hypertensive rats [Abstract]. Clin Res 31(2):336A

    Google Scholar 

  45. Strazzullo P, Nunziata V, Cirillo M et al. (1983) Abnormalities of calcium metabolism in essential hypertension. Clin Sci 65:137

    Google Scholar 

  46. Tsien RY, Pozzan T, Rink TJ (1982) Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new intracellular trapped fluorescent indicator. J Cell Biol 94:325

    Google Scholar 

  47. Webb RC, Bohr DF (1981) Recent advances in the pathogenesis of hypertension: consideration of structural, functional, and metabolic vascular abnormalities resulting in elevated arterial resistance. Am Heart J 251

  48. Wright GL, Rankin GO (1982) Concentrations of ionic and total calcium in plasma of four models of hypertension. Am J Physiol 243:H365

    Google Scholar 

  49. Wright GL, Toraason MA, Barbe JS, Crouse W (1980) The concentrations of ionic and total calcium in plasma of the spontaneously hypertensive rat. Can J Physiol Pharmacol 58(12):1494

    Google Scholar 

  50. Zidek W, Vetter H, Zumkley H, Losse H (1982) Intracellular Na and Ca activities in essential hypertension. Clin Sci 63:415

    Google Scholar 

  51. Zimlichman R, Goldstein DS, Zimlichman S, Keiser HR, Cytosolic calcium in plateles of spontaneously hypertensive rats. J Hypertension 4 (1986):283

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Internal Medicine, Yale University, Division of Endocrinology, New Haven

    H. Haller

  2. I. Medizinische Klinik, Städtische Kliniken Karlsruhe, Germany

    Th. Philipp

Authors
  1. H. Haller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Th. Philipp
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Diese Arbeit wurde unterstützt durch die Deutsche Forschungsgemeinschaft (DFG-Projekt Ha-1388/2-1)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Haller, H., Philipp, T. Intrazelluläres freies Kalzium und Plasma-Kalzium bei Patienten mit essentieller Hypertonie. Klin Wochenschr 66, 455–461 (1988). https://doi.org/10.1007/BF01745518

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01745518

Key words

Search

Navigation