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Topographical relationships between catecholamine-and neuropeptide-containing fibers in the median eminence of the newt,Triturus alpestris

An ultrastructural immunocytochemical study

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Summary

Dopaminergic and peptidergic nerve fibers were simultaneously demonstrated with a double-labeling technique at the ultrastructural level. The first antibody, raised against tyrosine hydroxylase, was applied during the preembedding phase and visualized with the peroxidase method. The second antibody, raised against one of the peptides met-enkephalin, somatostatin or gonadotropin-releasing hormone (GnRH), was applied to the ultrathin sections and visualized with gold-labeled goat anti-rabbit IgG. The fibers of both categories were present in the zona externa of the median eminence, frequently contacting the basal lamina of the portal vessels. In addition, topographical relationships between different types of nerve fibers were observed in the perivascular areas, although there were no morphological signs of synaptic specializations. Using serial sections, it could be established that one GnRH-fiber contacted both a dopaminergic fiber and a fiber immunoreactive for met-enkephalin. The observations support earlier physiological data concerning the regulation of the hypothalamo-hypophyseal axis, with special emphasis on the release of neurohormones in the median eminence of the newt.

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Authors and Affiliations

  1. CNRS, Centre de Neurochimie, Antenne Cronenbourg, BP 20 CR, F-67037, Strasbourg cedex, France

    M. Corio

  2. College de France, Laboratoire de Biochimie Cellulaire, Paris, France

    J. Thibault

  3. Department of Experimental Zoology, University of Utrecht, Utrecht, The Netherlands

    J. Peute

Authors
  1. M. Corio
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  2. J. Thibault
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  3. J. Peute
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Corio, M., Thibault, J. & Peute, J. Topographical relationships between catecholamine-and neuropeptide-containing fibers in the median eminence of the newt,Triturus alpestris . Cell Tissue Res. 259, 561–566 (1990). https://doi.org/10.1007/BF01740784

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