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Parathion exposure studies. A gas chromatographic method for the determination of low levels of p-nitrophenol in human and animal urine

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Summary

A method for the determination of low levels of PNP in rat and human urine was developed. The method involves acid hydrolysis of urine, extraction with benzene, derivatization of PNP with diazoethane, silica gel column chromatographic cleanup and gas chromatographic determination of the ethyl ether of PNP using an electron capture detector. Recoveries of fortified rat and human urine samples averaged in the range of 85–97%. The limits of detectability were 0.05 ppm for rat urine and 0.02 ppm for human urine. The method was applied to the analysis of PNP in urine of rats exposed to 0.1 and 0.01 LD50 of parathion.

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Authors and Affiliations

  1. Perrine Primate Laboratory, Environmental Protection Agency, P.O. Box 490, 33157, Perrine, Fla.

    Diane E. Bradway & T. M. Shafik

Authors
  1. Diane E. Bradway
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  2. T. M. Shafik
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Bradway, D.E., Shafik, T.M. Parathion exposure studies. A gas chromatographic method for the determination of low levels of p-nitrophenol in human and animal urine. Bull. Environ. Contam. Toxicol. 9, 134–139 (1973). https://doi.org/10.1007/BF01684992

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  • DOI: https://doi.org/10.1007/BF01684992

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