Summary
The behavior of plastid and mitochondrial nuclei (synonymous with nucleoids) during spermatogenesis inChara corallina was examined by fluorescence microscopy after staining with 4′,6-diamidino-2-phenylindole (DAPI). These organelle nuclei, which were present in internode cells and cells at the early spermatid stage, disappeared during spermatogenesis. This conclusion was confirmed by immunofluorescence microscopy using of a monoclonal anti-DNA antibody. The pattern of fluorescence obtained using the antibody coincided with that obtained by staining with DAPI. These results suggest that the disappearance of nuclei from male-derived organelles is most likely to result in maternal inheritance inChara corallina.
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Sun, G.H., Uyeda, T.Q.P. & Kuroiwa, T. Destruction of organelle nuclei during spermatogenesis inChara corallina examined by staining with DAPI and anti-DNA antibody. Protoplasma 144, 185–188 (1988). https://doi.org/10.1007/BF01637252
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DOI: https://doi.org/10.1007/BF01637252