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Immunofluorometric determination of antigenic differences between whole-cell antigens ofLegionella pneumophila

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Abstract

The immunofluorometric procedure has been utilized to assess the relative antigenicity of whole-cell antigens ofLegionella pneumophila. Observed response curves and interpretations of them are presented with experimental results that directly support the interpretation of such reaction curves. Application of the method for evaluating the relative antigenicity of strains maintained in tissue versus laboratory strains, routinely transferred on Feeley-Gorman or charcoal yeast extract agar, showed that laboratory strains have quantitatively gained, rather than lost, surface antigens directly involved in the human immune response to infection.

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Authors and Affiliations

  1. Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control, 30333, Atlanta, GA, USA

    Leo Pine

  2. Biological Products Program, Center for Infectious Diseases, Centers for Disease Control, 30333, Atlanta, GA, USA

    Robert F. Benson

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  1. Leo Pine
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  2. Robert F. Benson
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Pine, L., Benson, R.F. Immunofluorometric determination of antigenic differences between whole-cell antigens ofLegionella pneumophila . Current Microbiology 8, 255–261 (1983). https://doi.org/10.1007/BF01577724

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