Abstract
Prototrophic RD mutant cells ofCandida utilis NRRL-Y-1084 and auxotrophic mutant respiratory-competent cells ofSaccharomyces cerevisiae 4003-5Ba his 4 leu 2 can S meth 2 trp 5 ade 1 ura 3 gal were turned into protoplasts to be further fused with the aid of polyethylene glycol (PEG) and Ca2+ ions. Minimal medium containing glycerol as the carbon source was employed for fusion product selection. The respiratory-competent fusion products, mainly oval cells, resembledCandida utilis and had the fermentative abilities of this strain (dextrose, sucrose, raffinose). Five fusion products were analyzed as to their ability to metabolize dextrose, xylose, cellobiose, trehalose, glycerol, succinic acid, citric acid, salycin, and maltose. Fusion products partially restored the respiratory-competentCandida utilis capacity to grow by use of these carbon compounds, and none of theSaccharomyces cerevisiae fermenting abilities were found. Our results would suggest either a partial recombination between parental mitochondria or some occurring phenomenon affecting the cell, membrane function after somatic fusion without concomitant nuclear fusion.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Allmark, B. M., Morgan, A. J., Whittaker, P. A. 1978. The use of protoplast fusion in demonstrating chromosomal and mitochondrial inheritance of respiratory-deficiency inKluyveromyces lactis, apetite negative yeast. Molecular and General Genetics159:297–299.
Ferenczy, L., Maráz, A. 1977. Transfer of mitochondria by protoplast fusion inSaccharomyces cerevisiae. Nature268:524–525.
Fukuda, H., Kimura, A. 1980. Transfer of mitochondria into protoplasts ofSaccharomyces cerevisiae by mini-protoplast fusion. FEBS Letters113:58–60.
Gunge, N. 1979. Fusion of mitochondria with protoplasts inSaccharomyces cerevisiae. Molecular and General Genetics170:243–247.
Lancashire, W. E., Matoon, J. R. 1979. Genetic manipulation of a latent defect in yeast cytochrome biosynthesis utilizing cytoduction. Biochemical and Biophysical Research Communications90:801–809.
Lodder, J. 1970. Criteria and methods used in classification, pp. 65–83. In: Lodder J. (ed.), The yeast. Amsterdam: North Holland.
Maráz, A., Ferenczy, L. 1980. Selective transfer of fungal cytoplasmic genetic elements by protoplast fusion. Current Microbiology4:343–344.
Maráz, A., Kiss, M., Ferenczy, L. 1978. Protoplast fusion inSaccharomyces cerevisiae strains of identical and opposite mating types. FEBS Letters3:319–322.
Maráz, A., Subik, J. 1981. Transmission and recombination of mitochondrial genes inSaccharomyces cerevisiae after protoplast fusion. Molecular and General Genetics181:131–133.
Matsuoka, M., Uchida, K., Aiba, S. 1982. Cytoplasmic transfer of oligomycin resistance during protoplast fusion ofSaccharomycopsis lipolytica. Journal of Bacteriology151:530–533.
Morgan, A., Heritage, J., Whittaker, P. A. 1977. Protoplast fusion betweenpetite and auxotrophic mutants of thepetite-negative yeast,Kluyveromyces lactis. Microbios Letters4:103–107.
Ogur, M., St. John, R., Nagai, S. 1957. Tetrazolium overlay technique for population studies of respiratory deficiency in yeast. Science125:928–930.
Richard, M., Figueroa, L., van Broock, M. 1983. “Petitelike” mutants of the petite-negative yeastCandida utilis after treatment with ethidium bromide. Current Microbiology8:201–204.
Sarachek, A., Rhoads, D. 1981. Production of heterokaryons ofCandida albicans by protoplast fusions: effects of differences in proportions and regenerative abilities of fusion partners. Current Genetics4:221–222.
Sarachek, A., Rhoads, D., Schwarzhoff, R. H. 1981. Hybridization ofCandida albicans through fusion of protoplasts. Archives of Microbiology129:1–8.
Sipiczki, M., Ferenczy, L. 1977. Protoplast fusion ofSchizosaccharomyces pombre auxotrophic mutants of identical mating types. Molecular and General Genetics151:77–81.
Spencer, J. F. T., Land, P., Spencer, D. M. 1980. The use of mitchondrial mutants in the isolation of hybrids involving industrial yeast strain. II. Use in isolation of hybrids obtained by protoplast fusion. Molecular and General Genetics178:651–654.
Spencer, J. F. T., Spencer, D. 1981. The use of mitochondrial mutants in hybridization of industrial yeasts. III. Restoration of mitochondrial function in petites of industrial yeast strains by fusion with respiratory-competent protoplasts of other yeast species. Current Genetics4:177–180.
Stahl, U. 1978. Zygote formation and recombination between like mating types in the yeastSaccharomycopsis lipolytica by protoplast fusion. Molecular and General Genetics160:111–113.
Svoboda, A. 1978. Fusion of yeast protoplasts induced by polyethylene glycol. Journal of General Microbiology109:109–175.
Svoboda, A. 1981. Polyethylene glycol induced membrane fusion in yeast protoplasts. Acta Histochimica (Suppl. 23)S:211–217
van Broock, M. 1980. Genetic improvement and cell fusion in yeasts, PhD thesis, National University of Tucumán, Tucumán, Argentina.
van Broock, M., Sierra, M., Figueroa, L. 1981. Intergeneric fusion of yeast protoplasts. Proceedings of the Fifth International Symposium of Yeasts, pp. 171–176. In: Stewart, G. G., Russell, I. (eds.), Current developments in yeast research, Willowdale, Canada: Pergamon.
Yamashita, K., Fukuda, H., Murata, K., Kimura, A. 1981. Transfer of mitochondria ofHansenula wingeii into protoplasts ofSaccharomyces cerevisiae by mini-protoplast fusion.132:305–307.
Yoshida, K. 1979. Interspecific and intraspecific mitochondria-induced cytoplasmic transformation in yeasts. Plant and Cell Physiology20:851–856.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de Richard, M.S., de van Broock, M.R. Protoplast fusion between a petite strain ofCandida utilis andSaccharomyces cerevisiae respiratory-competent cells. Current Microbiology 10, 117–120 (1984). https://doi.org/10.1007/BF01576769
Issue Date:
DOI: https://doi.org/10.1007/BF01576769