Abstract
The primary anti-NP response in C57BL/6 and CBA mice was analyzed by isoelectric focusing. Antibody responses in C57 mice were restricted in heterogeneity and over 80 % of this strain shared an isoelectric focusing spectrum characteristic of an homogeneous antibody (spectrotype). This spectrotype N-l was inherited as a dominant characteristic in (CBA×C57)F1 mice, and backcross analysis revealed that it was genetically linked to the heavy chain allotypeIg-1 b. It thus behaved as a marker similar to the fine specificity characteristic of heteroclitic antibody. Elution studies showed that antibody with the spectrotype N-1 was heteroclitic, with a higher affinity for NIP and NNP than for the immunogen NP. It is argued that a) a single germ-lineV gene (designated N-1) codes for the VH region of this antibody and b) in C57BL/6 mice this geneV H N-1 is closely linked toIg-1 b.
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Abbreviations
- BSA:
-
bovine serum albumin
- CG:
-
Chicken globulin
- NP:
-
(4-Hydroxy-3-nitrophenyl)acetyl
- NIP:
-
(4-Hydroxy-5-iodo-3-nitrophenyl)acetyl
- NBrP:
-
(4 Hydroxy-5-bromo-3-nitrophenyl)acetyl
- NNP:
-
(4-Hydroxy-3,5-dinitrophenyl)acetyl
- NIP-Cap:
-
NIP-aminocaproate
- I50 :
-
Hapten concentration causing 50% inhibition of antigen binding
- SRC:
-
Sheep red cells
- IEF:
-
Isoelectric focusing
- PBS:
-
Phosphate buffered saline, pH 7.4
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McMichael, A.J., Phillips, J.M., Williamson, A.R. et al. Inheritance of an isoelectrically focused spectrotype linked to theIg-1 b allotype. Immunogenetics 2, 161–173 (1975). https://doi.org/10.1007/BF01572284
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DOI: https://doi.org/10.1007/BF01572284