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Spiroplasmavirus group 1: Isolation, growth, and properties

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Abstract

A group S1 spiroplasmavirus, SV1/KC3·BC3, from spiroplasma strain KC3, was propagated on spiroplasma strain BC3 and purified by density gradients. Purified virus had a density of 1.21 g/cm3 in metrizamide and 1.39 g/cm3 in CsCl and contained DNA of undetermined size and conformation. The virus was stable to exposure to non-ionic detergents, pH's between 6 and 9, heating at 60°C, drying, and (partially) ether; but was sensitive to pH extremes, chloroform, and 100°C. One-step growth experiments showed, by plaque assay, virus release beginning at 60 min and continuing for at least 6 h with no decrease in host cell growth. Infection was thus nonlytic, but the occurrence of 2-mm turbid plaques on agar indicates either belated host cell death or decreased division rate. The virus differed in some of these respects from the morphologically similar group 1 acholeplasmaviruses.

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Authors and Affiliations

  1. Department of Health and Human Services, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, 59840, Hamilton, Montana, USA

    Alan Liss

  2. Laboratory of Streptococcal Diseases, 20205, Bethesda, Maryland, USA

    Roger M. Cole

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  1. Alan Liss
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  2. Roger M. Cole
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Liss, A., Cole, R.M. Spiroplasmavirus group 1: Isolation, growth, and properties. Current Microbiology 5, 357–362 (1981). https://doi.org/10.1007/BF01566749

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