Abstract
Rabbit anti-mouse-Ia serum was raised against Ia specificities present in CBAJH (H-2 k) serum. This xenogeneic antiserum was considered to react with similar specificities to those detected by mouse anti-Iak alloantisera and more evidence is now presented for this contention. By absorption, the xenogeneic antiserum was found to react with spleen, lymph node, bone marrow, and thymus, reactions similar to that found with the allogeneic anti-Iak antiserum. Furthermore, red cells, platelets, brain, kidney, and liver could not absorb the activity from the xenogeneic antiserum, demonstrating the selective tissue distribution of the antigens reactive with this serum. This reactive population was previously shown to consist of B cells and a subpopulation of T cells. In a backcross study of (C57BL/6 × A)F1 × C57BL/6, the rabbit anti-Ia and mouse anti-Ia reactions were found to segregate together, and some evidence for the genetic regulation of the expression of Ia specificities was also found. By direct testing, and by absorption testing using a number of strains, the xenogeneic antiserum was shown to contain high titers of antibody to Ia.1′, 3′, 7′, 15′, and 17′; lower titers to Ia.19′, and 22′; little antibody to Ia.18′, and no reaction for the private specificity Ia. 2, although the multiple absorptions required to define these specificities may have observed some reactions. The data indicate that the xenogeneic and allogeneic anti-Iak antisera recognize similar Ia determinants, which map to theLA, IE andIC subregions of theH-2 complex. These have been given the same specificity designation as the allogeneic specificities, but they are separately identified by a prime (').
Similar content being viewed by others
References
Dain, A.R. and Hall, J.G.: A method for the isolation of white cells from the blood of sheep by differential lysis with hyptonic saline solution.Vox Sang. 13:281–284, 1967
David, C.S.: Serological and genetic aspects of murine Ia antigens.Transplant. Rev. 30:299–330, 1976
David, C.S., Stimpfling, J.H., and Shreffler, D.C.: Identification of specificity H-2.7 as an erythrocyte antigen: control by an independent locus,H-2G, between theS andD regions.Immunogenetics 2:131–139, 1975
Hämmerling, G.J.: Tissue distribution of Ia antigens and their expression on lymphocyte sub-populations.Transplant. Rev. 30:64–82, 1976
Klein, J., Hauptfeld, V., and Hauptfeld, M.: Evidence for a fifth (G) region in theH-2 complex of the mouse.Immunogenetics 2:141–150, 1975
McKenzie, I.F.C., Clarke, A.E., and Parish, C.R.: Ia antigenic specificities are oligosaccharide in nature: hapten inhibition studies.J. Exp. Med. 145:1039–1054, 1977
Murphy, D.B., Hertzenberg, L.A., Okumura, K., and McDevitt, H.O.: A newI region (IJ) marked by a locus (Ia−4) controlling surface determinants on suppressor T lymphocytes.J. Exp. Med.144:699–712, 1976
Parish, C.R., Kirov, S.M., Bowern, N., and Blanden, R.V.: A one-step procedure for separating mouse T and B lymphocytes.Eur. J. Immunol. 4:808–815, 1974
Parish, C.R., Chilcott, A.B., and McKenzie, I.F.C.: Low molecular-weight Ia antigens in normal mouse serum. I. Detection and production of a xenogeneic antiserum.Immunogenetics 3:113–128, 1976a
Parish, C.R., Chilcott, A.B., and McKenzie, I.F.C.: Low molecular-weight Ia antigens in normal mouse serum. II. Demonstration of their T-cell origin.Immunogenetics 3:129–137, 1976b
Parish, C.R., Jackson, D.C., and McKenzie, I.F.C.: Low molecular-weight Ia antigens in normal mouse serum. III. Isolation and partial chemical characterization.Immunogenetics 3:455–463, 1976c
Parish, C.R., Jackson, D.C., and McKenzie, I.F.C.: Evidence that Ia specificities are defined by carbohydrate.Proc. 3rd Ir Gene Workshop (in press) 1977
Shreffler, D. and David, C.S.: TheH-2 major histocompatibility complex and theI immune response region: genetic variation function, and organization.Adv. Immunol. 20:125–187, 1975
Shreffler, D.C., David, C.S., Cullen, S.E., Frelinger, J.A., and Neiderihuber, J.E.: Serological and functional evidence for further subdivision of theI region of theH-2 gene complex.The 41st Cold Spring Harbor Symposium 41:477–487, 1977
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Parish, C.R., McKenzie, I.F.C. A detailed serological analysis of a xenogeneic anti-Ia serum. Immunogenetics 6, 183–196 (1978). https://doi.org/10.1007/BF01563908
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01563908