Abstract
The technique of cell-surface iodination, followed by immuno-precipitation withPC-1.1 antiserum, was applied to normal spleen cells and to MOPC-70A BALB myeloma cells. The results indicate that the cell-surface component bearing PC-1 alloantigen has a molecular weight of from 105,000 to 110,000 daltons and does not resemble any constituent of plasma membranes or MuLV-type virus so far categorized by similar methods. The PC-1 specificity of the molecule was confirmed by comparison of the spleen cells of two mouse strains with spleen cells of their respective PC-congenic partner strains. MOPC-70A myeloma cells, but not spleen cells, yield a fainter band in the PC-1 position in controls in which antiserum is omitted, but peptide maps show that this similarly placed band has no relation to the PC-1 molecule.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- PC:
-
plasma cell
- B6:
-
C57BL/6
- SDS/PAGE:
-
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
- a:
-
anti
- NMS:
-
normal mouse serum
- MuLV:
-
murine leukemia virus
- Ig:
-
immunoglobulin
References
Barclay, A.N., Letarte-Muirhead, M., Williams, A.F., and Faulkes, R.A.: Chemical characterization of the Thy-1 glycoproteins from the membranes of rat thymocytes and brain.Nature 263:563–567, 1976
Durda, P.J. and Gottleib, P.D.: The Ly-3 antigens on mouse thymocytes: immune precipitation and molecular characterization.J. Exp. Med. 144:476–493, 1976
Elder, J.H., Jensen, F.C., Bryant, M.L., and Lerner, R.A.: Polymorphism of the major envelope glycoprotein (gp70) of murine C-type viruses: virion associated and differentiation antigens encoded by a multi-gene family.Nature 267:23–28, 1977
Fowler, A.V. and Zabin, I.: The amino acid sequence ofβ-galactosidase of Escherichia coli.Proc. Natl. Acad. Sci. U.S.A. 74:1507–1510, 1977
Laemmli, U.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227:680–685, 1970
Schwartz, B.D., Kask, A.M., Sharrow, S.O., David, C.S., and Schwartz, R.H.: Partial chemical characterization of Ia antigens derived from murine thymocytes.Proc. Natl. Acad. Sci. U.S.A. 74:1195–1199, 1977
Takahashi, T., Old, L.J., and Boyse, E.A.: Surface alloantigens of plasma cells.J. Exp. Med. 131:1325–1341, 1970
Tung, J.-S., Vitetta, E.S., Fleissner, E., and Boyse, E.A.: Biochemical evidence linking the GIX thymocytes surface antigen to the gp69/71 envelope glycoprotein of murine leukemia virus.J. Exp. Med. 141:198–205, 1975
Tung, J.-S., Pinter, A., and Fleissner, E.: Two species of type C viral core polyproteins on AKR mouse leukemia cells.J. Virol. 23:430–435, 1977a
Tung, J.-S., Michaelson, J., Sato, H., Vitetta, E.S., and Boyse, E.A.: Properties of the Lyb-2 molecule.Immunogenetics, 5:485/488, 1977b
Vitetta, E.S., Baur, S., and Uhr, J.W.: Cell surface immunoglobulin. II. Isolation and characterization of immunoglobulin from mouse splenic lymphocytes.J. Exp. Med. 134:242–264, 1971
Vitetta, E.A., Uhr, J.W., and Boyse, E.A.: Isolation and characterization of H-2 and TL alloantigens from the surface of mouse lymphocytes.Cell. Immunol. 4:187–191, 1972
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tung, J.S., Shen, F.W., Boyse, E.A. et al. Properties of the PC-1 molecule. Immunogenetics 6, 101–105 (1978). https://doi.org/10.1007/BF01563900
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01563900