Abstract
Rabbit anti-Ia sera was produced by immunization with detergentsolubilized extracts from splenic, lymph-node and thymus cells. The antisera contained activity against H-2 as well as Ia molecules. By a sequential immunoprecipitation assay it was shown that the rabbit anti-mouseH-2 s serum precipitated a second Ia molecule in theH-2 s haplotype. Previous studies with alloantisera have shown only one Ia molecule associated with this haplotype. Sequential precipitations with alloantiserum against the wholeI region were used to show that this second Ia molecule is coded by genes within theI region. Since only I-A- and I-E-region coded molecules are immunoprecipitable in most haplotypes, we presume that the rabbit antiserum could be identifying the I-E-subregion coded molecule in theH-2 s haplotype. The rabbit antiserum reacts with an isotypic specificity on the molecule. The studies suggest that theI-E subregion does exist in theH-2 s haplotype even though alloantiserum cannot be produced to identify allotypic variants associated with this subregion.
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Cook, R. G., Vitetta, E. S., Uhr, J., and Capra, J. D.: Structural studies on the murine Ia alloantigens. V. Evidence that the structural gene for the I-E/C beta polypeptide is encoded within the I-A subregion.J. Exp. Med. 149: 981–986, 1979
Cullen, S. E., Freed, J. H., and Nathenson, S. G.: Structural and serological properties of murine Ia alloantigens.Transplant. Rev. 30: 236–239, 1976
Cullen, S. E. and Schwartz, B. D.: An improved method for isolation of H-2 and Ia alloantigens with immunoprecipitation induced by Protein-A bearing staphylococci.J. Immunol. 117: 136–142, 1976
David, C. S. and Cullen, S. E.: Murine Ia antigens: identification and mapping of Ia.23 and further definition of theI-E subregion.J. Immunol. 120: 1659–1662, 1978
David, C. S., Beisel, K. W., Krco, C. J., and Lafuse, W.: Genetic organization, tissue expression and functional role of murine Ia antigens.In R. A. Reisfeld (ed.):Current Trends in Histocompatibility, Plenum Press, New York, in press, 1980
Jones, P. P., Murphy, D. B., and McDevitt, H. O.: Two gene control of the expression of a murine Ia antigen.J. Exp. Med. 148: 125–939, 1978
Jones, P. P., Murphy, D. B., and McDevitt, H. O.: Variability inI-A antigen expression.Fed. Proc. 38: 1280, 1979
McMillan, M., Cecka, J. M., Murphy, D. B., McDevitt, H. O., and Hood, L.: Structure of murine Ia antigens: partial NH-2-terminal amino acid sequences of products of theI-E orI-C subregions.Proc. Natl. Acad. Sci. U.S.A. 74: 5133–5139, 1977
Sachs, D. H., El-Gamil, M., Kiszkiss, P., Lunney, J. K., Mann, D. L., Ozato, K., and Shinohara, M.: Ia antigen cross-reactions between species.In F. H. Bach, B. Bonavida and E. S. Vitetta (eds.):T and B Lymphocyte Recognition and Function, Academic Press, New York, in press, 1980
Shreffler, D. C., David, C. S., Cullen, S. E., Frelinger, J. A., and Niederhuber, J. E.: Serological and functional evidence for further subdivision of the I regions of the H-2 gene complex.Cold Spring Harbor Symp. Quant. Biol. 41: 422–487, 1977
Silver, J., Russel, W. A., Reis, B. L., and Frelinger, J. A.: Clinical characterization of murine Ia alloantigens determined by theI-E/I-C subregions ofH-2 complex.Proc. Natl. Acad. Sci. U.S.A. 74: 5131–5134, 1977
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Lafuse, W.P., Neely, B.C. & David, C.S. Biochemical characterization of murine Ia antigens with xenoantisera. Immunogenetics 10, 311–318 (1980). https://doi.org/10.1007/BF01561581
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DOI: https://doi.org/10.1007/BF01561581