Skip to main content
SpringerLink
Log in

Maintenance of respiratory control in mitochondria after rate zonal centrifugation

  • Published:
Journal of bioenergetics Aims and scope Submit manuscript

Abstract

The respiratory control of rat liver mitochondria is lost when they are subjected to rate zonal centrifugation in a sucrose gradient (8.0% to 46.6%, w/w) at values for ω2t necessary for resolution. High sucrose concentration and high ω2t are both responsible. Respiratory control can be maintained in iso-osmotic Ficoll + 8.3% sucrose media, and after zonal centrifugation in such media at values of ω2t sufficient for resolution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C. de Duve,J. Cell Biol.,50 (1971) 20.

    Google Scholar 

  2. R. Wattiaux, S. Wattiaux-DeConinch, and M-F. Ronveaux-Dupal,Eur. J. Biochem.,22 (1971) 31.

    PubMed  Google Scholar 

  3. C. A. Price, in “Manometric Techniques”, W. W. Umbreit, R. H. Burris and J. F. Stauffer (Burgess Pub. Co., Minneapolis) 5th ed. in press.

  4. D. W. Allmann, T. Wakabayashi, E. F. Korman, and D. E. Green,J. Bioenergetics 1 (1970) 73.

    Google Scholar 

  5. V. R. Potter,J. Biol. Chem.,169 (1947) 17.

    Google Scholar 

  6. E. Carafoli and A. L. Lehninger, in “Methods in Enzymology”, Vol. X, eds. R. W. Estabrook and M. E. Pullman, Academic Press, New York (1967) 745.

    Google Scholar 

  7. A. L. Lehninger,Physiol. Rev.,42 (1962) 467.

    PubMed  Google Scholar 

  8. C. V. Lusena and F. Depocas,Can. J. Biochem. 44 (1966) 497.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Departments of Biological Chemistry and Internal Medicine, The University of Michigan Medical School, 48104, Ann Arbor, Michigan

    M. J. Dimino (NIH Postdoctoral Fellow) & F. L. Hoch

Authors
  1. M. J. Dimino
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. L. Hoch
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dimino, M.J., Hoch, F.L. Maintenance of respiratory control in mitochondria after rate zonal centrifugation. J Bioenerg Biomembr 3, 525–529 (1972). https://doi.org/10.1007/BF01539061

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01539061

Keywords

Search

Navigation