Abstract
The composition and content of acid soluble nucleotides in intact beef heart mitochondria and different submitochondrial particles have been studied.
It has been established that phosphorylating ETPH(Mg++, Mn++) and slightly phosphorylating ETP(KOH) fragments of inner mitochondrial membrane, which possess a similar morphological structure, have identical composition and almost the same content of acid soluble nucleotides. Both types of particles contain adenine nucleotides, nicotinamide and flavin coenzymes. The content of ATP in both types of particles amounts to 70–80! of that in intact mitochondria. Not less than three molecules of adenine nucleotides were found to be present in such particles per cytochromea in a respiratory chain.
If mitochondria are sonicated in the presence of EDTA or ETPH(Mg++, Mn++) is treated with urea, one can obtain nonphosphorylating particles of a quite different morphology and much lower content of adenine nucleotides. In the case of ETPH(EDTA), only one molecule of adenine nucleotides remains bound with the respiratory chain. The conclusion may be drawn that, if the structure of submitochondrial fragments is damaged or changed, the content of nucleotides decreases sharply.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
F. L. Crane, J. L. Glenn and D. E. Green,Biochim. Biophys. Acta,22 (1966) 475.
M. Hansen and A. L. Smith,Biochim. Biophys. Acta,81 (1964) 214.
R. E. Beyer, in:Methods in Enzymology, vol. X. R. W. Estabrook and M. E. Pulman (eds.), Acad. Press, New York, 1967, p. 186.
V. N. Lusikov, V. A. Saks and I. V. Beresin,Biochim. Biophys. Acta,22 (1970) 16.
V. K. Akimenko, I. B. Minkov and A. D. Vinogradov,Biokhimiya,36 (1971) 655.
Yu. Evtodienko and E. N. Mokhova, in:Mitochondria, Structure and Function, Moscow, “Nauka”, 1966, p. 95.
A. D. Gornall, C. G. Bardawill and M. M. David,J. Biol. Chem. 177 (1949) 751.
V. I. Drobishev, S. E. Mansurova and I. S. Kulaev,J. Chromatogr. 69 (1972) 317.
M. Klingenberg, in:Mitochondrial Structure and Compartmentation, E. Quagliariello, S. Papa, E. C. Slater and J. M. Tager (eds.), Adriatica Editrice, Bari, 1967, p. 320.
R. Beyer,Canad. J. Biochem.,46 (1968) 677.
V. P. Skulachev,Uspekhi Biologicheskoy khimii,6 (1964) 180.
V. P. Skulachev,Energy Accumulation in the Cell, Moscow, “Nauka”, 1969.
C. Cooper,Biochemistry,4 (1965) 335.
J. B. Chappel and A. R. Crofts,Biochem. J.,95 (1965) 393.
E. Racker,Mechanisms in Bioenergetics, Acad. Press, New York and London, 1965.
J. B. Warshaw, K. W. Lam, B. Nagy and D. R. Sanadi,Arch. Biochem. Biophys.,123 (1968) 385.
H. Holzer and W. Duntze,Ann. Rev. Biochem.,40 (1971) 345.
W. H. Walker and T. P. Singer,J. Biol. Chem.,245 (1970) 4224.
E. B. Kerney, J. I. Salach, W. H. Walker, R. Seng and T. P. Singer,Biochem. Biophys. Res. Commun.,42 (1971) 490.
M. Klingenberg,FEBS Letters,6 (1970) 145.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mansurova, S.E., Drobyshev, V.I. & Kulaev, I.S. Nucleotides of beef heart mitochondria and submitochondrial particles. J Bioenerg Biomembr 3, 499–507 (1972). https://doi.org/10.1007/BF01539058
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01539058