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Quantitation of the energetic state in mitochondria and submitochondrial vesicles with 8-anilino-1-naphthalene sulfonic acid

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Abstract

Some of the apparently anomalous findings made with the fluorescent probe 8-anilino-1-naphthalene sulfonic acid (ANS) have been reinvestigated using rat liver mitochondria. The results have been found compatible with current views on energy conservation.

The direction of fluorescence and proton flux changes under different conditions have been delineated. The relation of these results to consideration of membrane polarity and organization is discussed.

The reliability of ANS fluorescence changes in determining the level of energization of mitochondria and submitochondrial preparations is discussed.

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Abbreviations

ANS:

8-anilino-1-naphthalene sulfonic acid

ΔF E and ΔH+ E :

O2 dependent change in fluorescence and H+ in mitochondria and SMP

SMP:

submitochondrial preparation

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Author notes

  1. Y. Avi-Dor

    Present address: Department of Chemistry, Technion, Haifa, Israel

  2. K. Utsumi

    Present address: Department of Biochemistry, Cancer Institute, Okayama University Medical School, Okayama, Japan

Authors and Affiliations

  1. Department of Physiology-Anatomy, University of California, 94720, Berkeley, California

    Y. Avi-Dor, K. Utsumi & L. Packer

Authors
  1. Y. Avi-Dor
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  2. K. Utsumi
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  3. L. Packer
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Avi-Dor, Y., Utsumi, K. & Packer, L. Quantitation of the energetic state in mitochondria and submitochondrial vesicles with 8-anilino-1-naphthalene sulfonic acid. J Bioenerg Biomembr 1, 511–522 (1970). https://doi.org/10.1007/BF01517188

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  • DOI: https://doi.org/10.1007/BF01517188

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