Abstract
Mitochondrial oxidative phosphorylation is central for generating ATP and maintaining energy homeostasis in most eukaryotic cells. The ex vivo measurement of mitochondrial oxygen consumption rates in intact cells or isolated organelles is a valuable approach to assess mitochondrial bioenergetics in various experimental conditions. In this chapter, we describe several step-by-step protocols for measuring mitochondrial respiration in intact cells, permeabilized cells (in situ mitochondria), and isolated organelles using both Clark-type polarographic oxygen electrode devices and the newly developed oxygen-sensing fluorophore-based Seahorse technology.
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Acknowledgments
Guigas’ group is supported by funding from the European Federation for the Study of Diabetes (EFSD/Lilly Research Grant Fellowship), the Société Francophone du Diabète (SFD), and the Dutch Organization for Scientific Research (ZonMW TOP Grant 91214131). To the memory of Dr. Roland Favier and Pr. Xavier Leverve.
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Vial, G., Guigas, B. (2018). Assessing Mitochondrial Bioenergetics by Respirometry in Cells or Isolated Organelles. In: Neumann, D., Viollet, B. (eds) AMPK. Methods in Molecular Biology, vol 1732. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7598-3_18
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DOI: https://doi.org/10.1007/978-1-4939-7598-3_18
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