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Immunohistochemical dynamics of leukotoxin (9.10-eooxv-12-octadecenoic acid) in lungs of rats

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Abstract

This paper investigates the immunohistochemical dynamics of leukotoxin (9,10-epoxy-12-octadecenoic acid, LTx) in the lungs of rats exposed to hyperoxia with or without paraquat. The rats were treated with 100% oxygen or ambient air for 24. 48, 72 and 96 h in the presence or absence of a low or high dose paraquat (1,1′-di-methyl-4,4′-bipyridinium, PQ) injection. Immunostaining for LTx demonstrated positive reactions in the neutrophils that showed a progressive increase in intensity of staining with time in all groups exposed to 100% oxygen and in the group with high dose PQ, but the positive findings were weak in the group injected with low dose PQ only. We found the positive immunostaining reaction not only in neutrophils but also in alveolar macrophages. This indicates that LTx is produced by alveolar macrophages as well as by neutrophils depending on the treatment period under hyperoxic conditions, suggesting that LTx is an important chemical mediator in pulmonary diseases.

Zusammenfassung

Dieser Artikel untersucht die immunhistochemische Dynamik von Leukotoxin (9,10-Epoxy-12-octadecensäure, LTx) in den Lungen von Ratten, die einer Hyperoxie mit oder ohne Paraquat ausgesetzt waren. Die Ratten wurden behandelt mit 100% Sauerstoff oder Umgebungsluft für 24, 48, 72 und 96 Stunden mit oder ohne Injektion einer niedrigen oder hohen Dose Paraquat (1,1′-Dimethyl-4,4′-bipyridium, PQ). Die Immunfärbung für LTx zeigte positive Reaktionen in den Neutrophilen. Diese zeigten eine zunehmende Verstärkung der Färbungsintensität in Abhängigkeit der Zeit in allen Gruppen mit Exposition von 100% Sauerstoff und in der Gruppe mit hoher Dose PQ, jedoch waren die positiven Befunde in der Gruppe mit Injektion nur niedriger Dose PQ schwach. Wir fanden die positive Immunfärbungsreaktion nicht nur in Neutrophilen, sondern auch in Alveolarmakrophagen. Dies zeigt, daß LTx sowohl von den Alveolarmakrophagen als auch von den Neutrophilen, abhängig von der Behandlungszeitdauer unter hyperoxischen Bedingungen, hergestellt wird, was dafür spricht, daß LTx ein wichtiger chemischer Mediator in Lungenerkrankungen ist.

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Authors and Affiliations

  1. Department of Forensic Medicine, Faculty of Medicine, University of Tokyo, 113, Tokyo, Japan

    Wei-dong Zhang, Masataka Nagao, Takehiko Takatori, Kimiharu Iwadate, Yoshiyuki Itakura, Yoshihiro Yamada, Hirotaro Iwase & Tsuneaki Oono

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  1. Wei-dong Zhang
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  2. Masataka Nagao
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  3. Takehiko Takatori
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  4. Kimiharu Iwadate
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  5. Yoshiyuki Itakura
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  6. Yoshihiro Yamada
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  7. Hirotaro Iwase
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  8. Tsuneaki Oono
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Zhang, Wd., Nagao, M., Takatori, T. et al. Immunohistochemical dynamics of leukotoxin (9.10-eooxv-12-octadecenoic acid) in lungs of rats. Int J Leg Med 107, 174–178 (1995). https://doi.org/10.1007/BF01428400

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  • DOI: https://doi.org/10.1007/BF01428400

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