Summary
Four latosolic soils, varying in total nitrogen content from 0.050 to 0.098 per cent and in organic matter from 0.95 to 1.56 per cent, were collected from the farm of University College, Ibadan, Nigeria.
No change in nitrogen content was observed when the soils were kept moist in the greenhouse for four months. Where a legume (Centrosema pubescens) was grown gains of nitrogen in the soil—plant system equivalent to from 100 to 200 pounds nitrogen per acre — 6 inches deep were obtained. Where a grass (Eleusine coracana) was grown gains of nitrogen in the soil—plant system equivalent to from 100 to 130 lb. nitrogen per acre — 6 inches deep occurred in the two soils with low initial nitrogen levels. The possible origin of this nitrogen accretion is discussed. The data support, but do not prove, the hypothesis that it is due to non-symbiotic fixation in the soil.
In one of the soils showing a significant nitrogen gain under grass the presence of Azotobacter was not demonstrated and Beijerinckia occurred in only one of 24 samples examined. Thus, if non-symbiotic biological nitrogen fixation did occur, Clostridium and other micro-organisms not belonging to the Azotobacteriaceae must have been the agents of fixation involved. On the other hand, the presence of Azotobacter in the two soils in which no fixation occurred could easily be demonstrated.
It is suggested that non-symbiotic nitrogen fixation by soil micro-organisms under grass cover in the humid tropics may be of greater significance than has been believed in the past.
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References
Alexander, M., Introduction to Soil Microbiology. John Wiley & Sons, Inc., N.Y. (1961).
Bayliss, N. S., The thermochemistry of biological nitrogen fixation. Australian J. Biol. Sci.9, 364–370 (1956).
Becking, J. H., Nitrogen-fixing bacteria of the genusBeijerinckia in South African soils. Plant and Soil11, 193–206 (1959).
Becking, J. H., Studies on nitrogen-fixing bacteria of the genusBeijerinckia. I. Geographical and ecological distribution in soils. Plant and Soil14, 49–81 (1961).
Bremner, J. M., Determination of nitrogen in soil by the Kjehldahl method. J. Agr. Sci.55, 11–33 (1960).
Bremner, J. M. and Shaw, K., Denitrification in soil. I. Methods of investigation. J. Agr. Sci.51, 23–29 (1958).
Brown, M. E. and Metcalfe, G., Nitrogen fixation by a species ofPullularia. Nature180, 282 (1957).
Brown, R., Nitrogen fixation by the endophyte ofLolium. J. Agr. Sci.23, 527–540 (1933).
Chapman, H. D., Liebig, G. F., and Rayner, D. S., A lysimeter investigation of nitrogen gains and losses under various systems of cover cropping and fertilization. Hilgardia19, 57–128 (1949).
Delwiche, C. C. and Wijler, J., Non-symbiotic nitrogen fixation in soil. Plant and Soil7, 113–129 (1956).
Gel'tser, F. Y., Importance of biological nitrogen fixation in forming the organic substances of the soil. Agrobiologiya4, 588–594 (1961). Abstr. Soils and Fertilizers25, 42 (1962).
Greenland, D. J., Denitrification in some tropicals soils. J. Agr. Sci.58, 227–233 (1962).
Hino, S. and Wilson, P. W., Nitrogen fixation by facultativeBacillus. J. Bacteriol.75, 403–408 (1958).
Jaiyebo, E. O., The influence of five different cover treatments on soil fertility in a tropical rainforest environment. Unpubl. M. Sc. thesis, Univ. London (1961).
Jensen, V., Nitrogen fixation by strains ofAerobacter aerogenes. Physiol. Plantarum9, 130–136 (1956).
Jensen, V., A new nitrogen fixing bacterium from a Danish watercourse. Arch. Mikrobiol.29, 348–353 (1958).
Jones, E., Contribution of rainwater to the nutrient economy of soil in Northern Nigeria. Nature188, 432 (1961).
Metcalfe, G. and Chayen, S., Nitrogen fixation by soil yeasts. Nature174, 841–842 (1954).
Moore, A. W. and Abaelu, J. N., Non-symbiotic nitrogen fixation in a soil of the Nigerian rain-forest zone. Nature184, 75 (1959).
Parker, C. A., Non-symbiotic nitrogen-fixing bacteria in soil. II. Studies onAzotobacter. Australian J. Agr. Research6, 388–397 (1955).
Parker, C. A., Non-symbiotic nitrogen-fixing bacteria in soil. III. Total nitrogen changes in a field soil. J. Soil Sci.8, 48–59 (1957).
Paul, E. A. and Newton, J. D., Studies of aerobic non-symbiotic nitrogen-fixing bacteria. Can. J. Microbiol.7, 7–13 (1961).
Rovira, A. D., Plant root exudates in relation to the rhizosphere microflora. Soils and Fertilizers25, 167–172 (1962).
Ruinen, J., Occurrence ofBeijerinckia species in the “phyllosphere”. Nature177, 220–221 (1956).
Smith, H. V., A lysimeter study of the nitrogen balance in irrigated soils. Arizona Agr. Exp. Sta. Tech. Bull.102, 255–308 (1944).
Smith, H. W. and Weldon, M. D., A comparison of some methods for the determination of soil organic matter. Soil Sci. Soc. Am. Proc.5, 177–182 (1940).
Stevenson, G., Fixation of nitrogen by non-nodulated seed plants. Ann. Botany N.S.23, 622–635 (1959).
Vandecaveye, S. C. and Villanueva, B. R., Microbial activities in soil. I. Nitrogen fixation byAzotobacter and activity of various groups of microbes in Palouse silt loam. Soil Sci.38, 191–205 (1934).
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Moore, A.W. Nitrogen fixation in latosolic soil under grass. Plant Soil 19, 127–138 (1963). https://doi.org/10.1007/BF01347867
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DOI: https://doi.org/10.1007/BF01347867