Summary
The rubella virus is composed of RNA (2.4 per cent dry weight of the virus), proteins (74.8 per cent), carbohydrates (4:2.5 per cent of which are present as aminosugars, 1.5 per cent as neutral sugars) and lipids (18.8 per cent).
The analysis of fatty acids in rubella virus was done at the same time as the analysis of control cells and infected cells.
In the virus, the main fatty acids are: palmitic (26 per cent), stearic (15 per cent), oleic (15 per cent). Rubella virus differs from other togaviruses by the presence of fatty acids with odd-numbers of atoms of C (C15, C17, C19) which represent 23 per cent of total acids and of an hydroxyacid.
In the cells, the acids oleic, palmitic, stearic and linoleic represent 90 per cent of total fatty acids. The infection of the BHK21/13S cells by rubella virus leads essentially to an increase (35 per cent) of the amount of linoleic acid with a decrease of palmitic and oleic acids.
Similar content being viewed by others
References
Ashwell, G.: Colorimetric analysis of sugars. In:Colowick, S. P., Kaplan, N. O. (eds.), Methods in Enzymology. Vol. 3, p. 73–105. New York: Academic Press 1957.
Bardeletti, G., Kessler, N., Aymard-Henry, M.: Morphology, biochemical analysis and neuraminidase activity of rubella virus. Arch. Virol.49, 175–186 (1975).
Bardeletti, G., Gautheron, D. C.: Phospholipid and cholesterol composition of rubella virus and its host cell BHK21 grown in suspension cultures. Arch. Virol.52, 19–27 (1976).
Bardeletti, G., Tektoff, J., Gautheron, D.: Rubella virus maturation and production in two host cells systems. Intervirology11, 97–103 (1979).
Bardeletti, G.: Etude du virus de la rubéole et de ses relations avec la cellule hôte. Thèse de doctoarat d'Etat ès-Sciences. Université Claude Bernard, Lyon (1979).
Bardeletti, G.: Respiration and ATP level in BHK21/13S cells during the earliest stages of Rubella Virus replication. Intervirology8, 100–109 (1977).
Bonnissol, C., Sisman, J.: Etude au microscope électronique du site de maturation du virus de la rubéole. C.R. Séances Acad. Sci. Paris267, 1337–1340 (1968).
David, A. E.: Lipid composition of Sindbis virus. Virology46, 711–720 (1971).
Folch, J., Lees, H., Sloane-Stanley, G. H.: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem.226, 497–509 (1957).
Holmes, I. H., Wark, M. C., Warburton, M. F.: Is rubella an arbovirus? II. Ultrastructural morphology and development. Virology37, 15–25 (1969).
Horzinek, M. C.: Togaviruses, Ann. Med. Vet.122, 293–299 (1978).
Kääriäinen, L., Söderlund, H.: Structure and replication of α-viruses. Curr. Top. Microbiol. Immunol.82, 15–69 (1978).
Klenk, H. D., Choppin, P. W.: Lipids of plasma membranes of monkey and hamster kidney cells and of parainfluenza virions grown in these cells. Virology38, 255–268 (1969).
Liebhaber, H., Gross, P. A.: The structural proteins of rubella virus. Virology47, 684–693 (1972).
McCombs, R. M., Brunschwig, J. P., Rawls, W. E.: Morphology of rubella virus. Exp. mol. Pathol.9, 27–33 (1968).
Murrhy, F. A., Halonen, P. E., Harrison, A. K.: Electron microscopy of the development of rubella virus in BHK21 cells. J. Virol.2, 1223–1227 (1968).
Payment, P., Adjukovic, D., Pavilanis, V.: Le virus de la rubéole. II. Replication dans les cellules Vero et effects de l'atinomycine D et du cycloheximide. Canad. J. Microbiol.21, 710–717 (1975).
Porterfield, J. S., Casals, J., Chumakov, M. P., Gaidamovich, S. Ya., Hannoun, C., Holmes, I. H., Horzinek, M. C., Mussgay, M., Oker-Blom, N., Russel, P. R., Trent, D. W.: Togaviridae. Intervirology9, 129–148 (1978).
Reissig, J. L., Strominger, J. L., Leloir, L. F.: A modified colorimetric method for estimation ofN-acetylamino-sugars. J. biol. Chem.217, 959–966 (1955).
Renkonen, O., Kääriäinen, L., Simons, K., Gahmberg, C. G.: The lipid class composition of Semliki Forest Virus and of plasma membranes of the host cells. Virology46, 318–326 (1971).
Renkonen, O., Gahmberg, C. G., Simons, K., Kääriäinen, L.: The lipids of the plasma membranes and endoplasmic reticulum from cultures baby hamster kidney cells (BHK21). Biochim. biophys. Acta255, 66–78 (1972).
Schlenk, H., Gellerman, J. L.: Esterification of fatty acids with diazomethane on a small scale. Anal. Chem.32, 1412–1414 (1960).
Stoker, M., MacPherson, I.: Syrian hamster fibroblast cell line BHK21 and its derivatives. Nature203, 1355–1357 (1964).
von Bonsdorff, C. H., Vaheri, A.: Growth of rubella virus in BHK21 cells electron microscopy of morphogenesis. J. gen. Virol.5, 47–51 (1969).
Wagner, H., Hörhammer, L., Wolff, P.: Dünnschicht-Chromatographie von Phosphatiden und Glykolipiden. Biochem. Z.334, 175–181 (1961).
Author information
Authors and Affiliations
Additional information
With 3 Figures
Rights and permissions
About this article
Cite this article
Voiland, A., Bardeletti, G. Fatty acid composition of rubella virus and BHK 21/13S infected cells. Archives of Virology 64, 319–328 (1980). https://doi.org/10.1007/BF01320617
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01320617