Summary
Reverse transcriptase from foamy virus, strain H4188 was estimated and purified. The enzyme has the following characteristics: 1. The reaction utilized preferentially oligo (dT) poly (rA) as a primer-template; however, the synthetic primer-template oligo (dT) poly (dA) could also be used to some extent. 2. The reaction utilized oligo (dG) poly (rC) as a primer-template with very low efficiency. 3. The crude virus preparation had a detectable endogenous reaction using the four deoxyribonucleotides for DNA polymerization. 4. The cation requirement for the enzyme reaction was much more biased for Mn++ than for Mg++ ions. 5. The molecular weight of the partially-purified enzyme was estimated to be about 80,000. Aggregates of 240,000 daltons were also seen.
The activity of this enzyme was not inhibited by antisera against the reverse transcriptases of various type C RNA viruses, namely, feline endogenous leukemia virus, RD 114, Woolly simian sarcoma virus (SSV-1) and avian myeloblastosis virus (AMV). Antiserum against Rauscher leukemia virus (RLV) enzyme was marginally active against foamy virus enzyme, perhaps indicating a slight cross-reaction. The biochemical characteristics of foamy virus reverse transcriptase seemed to be very close to those of the type C RNA viruses, but the immunological reaction proved that the foamy virus reverse transcriptase was distinct from the others.
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References
Baltimore, D., Smoler, D.: Primer requirement and template specifity of the polymerase of RNA tumor viruses. Proc. Natl. Acad. Sci. U.S.A.68, 1507–1511 (1971).
Gallagher, R. E., Todaro, G. J., Smith, R. G., Livingston, D. M., Gallo, R. C.: Relationship between RNA-Directed DNA polymerase (reverse transcriptase) from human acute leukemia blood cells and primate type-C viruses. Proc. Nat. Acad. Sci. U.S.A.71, 1309–1313 (1974).
Gilden, R. V., Long, C. W., Hanson, M., Toni, R., Charman, H. P., Ozosalar, S., Miller, J. M., van der Maaten, M. J.: Characteristics of the major internal protein and RNA-dependent DNA-polymerase of bovine leukemia virus. J. gen. Virol.29, 305–314 (1975).
Hooks, J. J., Gibbs, C. J., Jr., Chou, S., Houk, R., Lewis, M., Gajdusek, D. C.: Isolation of a new simian foamy virus from a spider monkey brain culture. Infect. Immun.8, 804–813 (1973).
Hooks, J. J., Gibbs, C. J., Jr.: The foamy viruses. Bact. Review39, 169–185 (1975).
Howk, R. S., Rye, L. A., Killeen, L. A., Scolnick, E. M., Parks, W. P.: Characterization and separation of viral DNA polymerase in mouse milk. Proc. Nat. Acad. Sci. U.S.A.70, 2117–2121 (1973).
Hruska, F., Takemoto, K. K.: Biochemical properties of a hamster syncytium forming (“foamy”) virus. J. Nat. Cancer Inst.54, 601–605 (1975).
Hsiung, G. D.: Latent virus infections in primate tissue with special reference to simian viruses. Bact. Rev.32, 185–205 (1968).
Johnston, P. B.: Studies on simian foamy viruses and syncytium-forming viruses of lower animals. Lab. Animal Sci.24, 159–166 (1974).
Lewis, B. J., Abrell, J. W., Smith, R. G., Gallo, R. C.: DNA polymerases in human lymphoplastoid cells infected with simian sarcoma virus. Biochim. biophys. acta349, 148–160 (1974).
Lowry, O. H., Rosebrough, N. J., Farr, H. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951).
Parks, W. P., Todaro, G. I., Scolnick, E. M., Aaronson, S. A.: RNA dependent DNA polymerase in primate syncytium-forming (Foamy) viruses. Nature229, 258–260 (1971).
Parks, W. P., Todaro, G. J.: Biological properties of syncytium-forming (“Foamy”) Viruses. Virology47, 673–683 (1972).
Parks, W. P., Scolnick, E. M., Ross, J., Todaro, G. J., Aaronson, S. A.: Immunological relationship of reverse transcriptase from RNA tumor viruses. J. Virol.9, 110–115 (1972).
Rabin, H., Neubauer, R. H., Woodside, N. J., Cicmanec, J. L., Wallen, W. C., Lapin, B. A., Agrba, V. A., Yakoleva, L. A., Chuvirov, G. N.: Virological studies of baboon (Papio hamadryas) lymphoma: Isolation and characterization of foamy viruses. J. Med. Primatol.5, 12–22 (1976).
Rogers, N. G., Basnight, M., Gibbs, C. J., Jr., Gajdusek, D. C.: Latent viruses in chimpanzees with experimental Kuru. Nature216, 446–449 (1967).
Rossignol, J. M., Kress, M., de Vaux Saint Cyr, C.: Induction par la 5-bromo-2′-désoxyuridine d'un virus de type “foamy” inapparent dans des cellules de hamster transformées par le SV 40. C.R. Acad. Sci. Paris281 (Série D), 1145–1148 (1975).
Scolnick, E., Rands, E., Aaronson, S. A., Todaro, G. J.: RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements. Proc. Natl. Acad. Sci. U.S.A.67, 1789–1796 (1970).
Scolnick, E. M., Parks, W. P., Todaro, G. J.: Reverse transcriptase of primate viruses as immunological markers. Science177, 1119–1121 (1972).
Wu, A. M., Gallo, R. C.: Interaction between murine type-C virus RNA-directed DNA polymerase and rifamycin derivatives. Biochim. biophys. acta340, 419–436 (1974).
Wu, A. M., Gallo, R. C.: Reverse transcriptase. CRC Critical Reviews in Biochemistry, 289–347 (1975).
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Liu, W.T., Natori, T., Chang, K.S.S. et al. Reverse transcriptase of foamy virus. Archives of Virology 55, 187–200 (1977). https://doi.org/10.1007/BF01319905
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DOI: https://doi.org/10.1007/BF01319905