Summary
Infection of Aedes albopictus cells with Semliki Forest virus (SFV) leads to polykaryocyte formation below pH 6.2. This syncytium formation is accompanied by a decrease of the cellular ATP level. Addition of inhibitors of oxidative phosphorylation leads to a rapid, total depletion of ATP in infected cells at pH 6 and results in an inhibition of polykaryocyte formation. However, when cells were exposed for only a few minutes to pH 6 in the presence of the inhibitors and then kept at pH 7.2, the ATP level partially recovered to values sufficient for syncytium formation. Similar results were obtained after ATP depletion induced by 2-deoxyglucose. Thus, it can be concluded that SFV-induced syncytium formation is an ATP-dependent event.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Besterman JM, Low RB (1983) Endocytosis: a review of mechanisms and plasma membrane dynamics. Biochem J 210: 1–13
Davey J, Hurley SM, Warren G (1985) Reconstitution of an endocytic fusion event in a cell-free system. Cell 43: 643–652
Finkeldey W (1931) Über Riesenzellbefunde in den Gaumenmandeln, zugleich ein Beitrag zur Histopathologie der Mandelveränderungen im Maserninkubationsstadium. Virchows Arch Pathol Anat Physiol Klin Med 281: 323–329
Goldstein JL, Anderson RG, Brown MS (1979) Coated pits, coated vesicles and receptor-mediated endocytosis. Nature 279: 679–685
Guccion JG, Enzinger FM (1972) Malignant giant cell tumor of soft parts. Cancer 29: 1518–1529
Henle G, Deinhardt F, Girardi A (1954) Cytolytic effects of mumps virus in tissue cultures of epithelial cells. Proc Soc Exp Biol Med 87: 386–393
Hertel C, Coulter SJ, Perkins JP (1986) The involvement of cellular ATP in receptor mediated internalization of epidermal growth factor and hormone-induced internalization of α-adrenergic receptors. J Biol Chem 261: 5974–5980
Igarashi A (1978) Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses. J Gen Virol 40: 531–544
Knight DE, Baker PF (1982) Calcium-dependence of catecholamine release from bovine adrenal medullary cells after exposure to intense electric fields. J Membr Biol 68: 107–140
Koblet H, Kempf C, Kohler U, Omar A (1985) Conformational changes at pH 6 on the cell surface of Semliki Forest virus-infected Aedes albopictus cells. Virology 143: 334–336
Omar A, Flaviano A, Kohler U, Koblet H (1986) Fusion of Semliki Forest virus infected Aedes albopictus cells at low pH is a fusion from within. Arch Virol 89: 145–159
Okada Y (1962) Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich's ascites tumor cells. I. Microscopic observation of giant polynuclear cell formation. Exp Cell Res 26: 98–107
Okada Y (1962) Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich's ascites tumor cells. III. Relationship between cell condition and fusion reaction or cell degeneration reaction. Exp Cell Res 26: 119–128
Oshiro LS, Schieble JH, Lenette EH (1971) Electron microscopic studies of a coronavirus. J Gen Virol 12: 161–168
Poste G, Nicholson GL (1978) Membrane fusion. In: Cell surface reviews, vol. 5. Elsevier/North Holland, Amsterdam
Roizman B (1962) Polykaryocytosis. Cold Spring Harbor Symp Quant Biol 27: 327–342
Steinman RM, Mellman IS, Muller WA, Cohn ZA (1983) Endocytosis and recycling of plasma membrane. J Cell Biol 96: 1–27
Walts A (1983) Osteoclast-type giant cell tumor of the pancreas. Acta Cytol 27: 400–404
White J, Matlin K, Helenius A (1981) Cell fusion by Semliki Forest, influenza, and vesicular stomatitis virus. J Cell Biol 89: 674–679
Author information
Authors and Affiliations
Additional information
With 6 Figures
Rights and permissions
About this article
Cite this article
Kempf, C., Kohler, U., Michel, M.R. et al. Semliki Forest virus-induced polykaryocyte formation is an ATP-dependent event. Archives of Virology 95, 111–122 (1987). https://doi.org/10.1007/BF01311338
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01311338