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Phosphohydrolases in Turbellaria Phagocata sibirica

  • Comparative and Ontogenic Biochemistry
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Abstract

Activity and properties of two phosphohydrolases, nucleoside diphosphatase (NDPase) and 5′-nucleotidase, were assayed in subcellular fractions of turbellaria Phagocata sibirica Sabussov (Turbellaria, Planariidae). The highest activity of N D Pase was observed in microsomes while that of 5′-nucleotidase in mitochondria and cytosolic fractions (12 000 and 105 000 g). The correlation between the rates of the relevant enzymatic reactions and concentrations of substrates and metal ions as well as the impact of various effectors and divalent cations (Mg2+, Mn2+, Ca2+, Zn2+, Cu2+) on phosphohydrolase activities were studied.

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References

  1. Moczon, T., Histochemical studies on the enzymes of Hymenolepis diminuta. II. Nonspecific and specific phosphatases in oncospheres and cysticercoids, Acta Parasitol. Pol., 1973, vol. 21, pp. 99–106.

    CAS  Google Scholar 

  2. Moczon, T., Histochemical studies on the enzymes of Hymenolepis diminuta. IV. Nonspecific phosphatases in a mature parasite, Acta Parasitol. Pol., 1974, vol. 22, pp. 323–329.

    Google Scholar 

  3. Roy, T.K., Histochemical studies on Raillietina (Raillietina) johri (Cestoda: Davaineidae). I. Nonspecific and specific phosphatases, J. Helminthol., 1979, vol. 53, pp. 45–49.

    Article  CAS  PubMed  Google Scholar 

  4. Humiczewska, M., Some specific and non-specific phosphatases of the sporocyst of Fasciola hepatica. II Enzymes associated with the membrane transport, Folia Parasitol., 2002, vol. 49, pp. 221–226.

    Article  Google Scholar 

  5. Omar, M.S. and Raoof, A.M.S., Histochemical distribution of hydrolytic enzymes in adult Onchocerca fasciata (Filarioidea: Onchocercidae), Parasitol. Res., 1994, vol. 80, pp. 216–222.

    Article  CAS  PubMed  Google Scholar 

  6. Sharma, P.N. and Mandawat, S., Phosphohydrolases of Paramphistomum cervi (Digenea, Paramphistomatidae), Acta Parasitol. Pol., 1983, vol. 28, pp. 381–392.

    CAS  Google Scholar 

  7. Wang, W., Dail, J., Li, H., and Liang, G., Histochemical and enzyme-histochemical studies of Oncomelania hupensis (Gredler, 1881), the invermediate host of Schistosoma japonicum, Acta Parasitol., 2011, vol. 56, pp. 105–110.

    Article  CAS  Google Scholar 

  8. Roy, T.K., Distribution, functional significance of phosphatases in the bovine amphistome Ceylonocotyle scoliocoelium, Indian J. Exp. Biol., 1980, vol. 18, pp. 385–392.

    CAS  PubMed  Google Scholar 

  9. Seniuta, R., Cytochemical studies on some specific phosphatase activities in experimental trichinellosis of mice, Wiadom. Parasitol., 1975, vol. 21, pp. 683–688.

    CAS  Google Scholar 

  10. Parshad, V.R. and Guraya, S.S., Comparative histochemistry observations on the excretory system of helminth parasites, Z. Parasitenk., 1977, vol. 52, pp. 81–89.

    Article  CAS  PubMed  Google Scholar 

  11. Gounaris, K., Selkirk, M.E., and Sadeghi, S. J., A nucleotidase with unique catalytic properties is secreted by Trichinella spiralis, Mol. Biochem. Parasitol., 2004, vol. 136, pp. 257–264.

    Article  CAS  PubMed  Google Scholar 

  12. Failer, B.U., Braun, N., and Zimmermann, H., Cloning, expression and functional characterization of a Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase, J. Biol. Chem., 2002, vol. 277, pp. 36 978–36 986.

    Article  CAS  Google Scholar 

  13. Bogitsh, B.J. and Krupa, P.L., Schistosoma mansoni and Haematoloechus medioplexus: nucleosidediphosphatase location in tegument, Exp. Parasitol., 1971, vol. 30, pp. 418–425.

    Article  CAS  PubMed  Google Scholar 

  14. Strauss, O. and Graszynski, K., Isolation of plasma membrane vesicles from the gill epithelium of the crayfish Orconectes limosus Rafinesque and properties of the Na+/H+ exchanger, Comp. Biochem. Physiol., 1992, vol. 102A, pp. 519–526.

    Article  Google Scholar 

  15. Lazou, A., Adenylate metabolizing enzymes in invertebrate tissues, Comp. Biochem. Physiol., 1989, vol. 92B, pp. 175–180.

    CAS  Google Scholar 

  16. Pinto, R.M., Canales, J., Faraldo, A., Sillero, A., and Sillero, M.A.G., Cytosol 5′-nucleotidase from Artemia embryos. Purification and properties, Comp. Biochem. Physiol., 1987, vol. 86B, pp. 49–53.

    CAS  Google Scholar 

  17. Umemori-Aikawa, Y., An alkaline phosphatase in the clam Meretrix meretrix lusoria (Gmelin) with affinities for nucleotides, Comp. Biochem. Physiol., 1971, vol. 40B, pp. 347–358.

    Google Scholar 

  18. Kochetov, T.A., Metod opredeleniya neorganicheskogo fosfora. Prakticheskoe rukovodstvo po enzimologii (A Method for Determination of Inorganic Phosphorus. Practical Handbook on Enzymology), Moscow, 1980.

  19. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., Protein measurement with the Folin phenol reagent, J. Biol. Chem., 1951, vol. 193, pp. 265–275.

    CAS  PubMed  Google Scholar 

  20. Cornish-Bowden, E., Osnovy fermentativnoi kinetiki (Fundamentals of Enzyme Kinetics), Moscow, 1979.

  21. Fujisawa, K. and Yoshino, M., Activities of adenylate-degrading enzymes in muscles from vertebrates and invertebrates, Comp. Biochem. Physiol., 1987, vol. 86, pp. 109–112.

    Article  Google Scholar 

  22. Arch, J.R.S. and Newsholme, E.A., Activities and some properties of 5′-nucleotidase, adenosine kinase and adenosine deaminase in tissues from vertebrates and invertebrates in relation to the control of the concentration and the physiological role of adenosine, Biochem. J., 1978, vol. 174, pp. 965–977.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Burenina, E.A., Influence of antihelmintic drugs on 5′-nucleotidase activity in Bothriocephalus scorpii (Cestoda: Bothriocephalidae), Ross. Parazitol. Zh., 2014, vol. 30, pp. 48–54.

    Google Scholar 

  24. Burenina, E.A., Enzymes of carbohydrate and energy metabolism in the turbellaria Phagocata sibirica., J. Evol. Biochem. Physiol., 2004, vol. 40(4), pp. 298–304.

    Article  CAS  Google Scholar 

  25. Burenina, E.A., Activities and properties of glucose-6-phosphatases in turbellaria Phagocata sibirica and cestodes Bothriocephalus scorpii, J. Evol. Biochem. Physiol., 2009, vol. 45(5), pp. 571–578.

    Article  CAS  Google Scholar 

  26. Burenina, E.A., Activity and properties of fructose bisphosphatase in turbellaria Phagocata sibirica, J. Evol. Biochem. Physiol., 2011, vol. 47(3), pp. 251–259.

    Article  CAS  Google Scholar 

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Funding

This study was supported by the state budget funding; program no. AAAA-A18-118013090245-6.

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Authors and Affiliations

  1. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia

    E. A. Burenina

  2. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    M. I. Zhukovskaya

Authors
  1. E. A. Burenina
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  2. M. I. Zhukovskaya
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Correspondence to E. A. Burenina.

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All applicable international, national and institutional principles of handling and using experimental animals for scientific purposes were observed.

This study did not involve human subjects as research objects.

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Russian Text © The Author(s), 2019, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2019, Vol. 55, No. 4, pp. 249–254.

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Burenina, E.A., Zhukovskaya, M.I. Phosphohydrolases in Turbellaria Phagocata sibirica. J Evol Biochem Phys 55, 277–283 (2019). https://doi.org/10.1134/S0022093019040033

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  • DOI: https://doi.org/10.1134/S0022093019040033

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