Summary
Adenoviruses (AV), isolated from 138 children during the year 1981 in the Hannover area, were studied by DNA restriction analysis with the enzymes BamHI, Bg1II, BstEII, EcoRI, HindIII, KpnI, and SmaI and compared with the respective prototypes. Varying fragment patterns were depicted and genome types analyzed. Prototype-like strains of AV1 and 5 were not found. Types 2, 5, 1, 3, and 7 showed decreasing genetic variation in that order. Altered restriction sites were physically mapped on the genome; they appeared to be randomly distributed. The high genetic variability of AV2 and 5 is remarkable for this study population, limited in time and space.
Clinical and epidemiological data were also presented in relation to serotypes.
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References
Adrian T, Best B, Wigand R (1985) A proposal for naming adenovirus genome types, exemplified by adenovirus type 6. J Gen Virol 66: 2685–2691
Adrian T, Wadell G, Hierholzer JC, Wigand R (1986) DNA restriction analysis of adenovirus prototypes 1 to 41. Arch Virol 91: 277–290
Adrian T, Wigand R (1986) Adenovirus 3–7, an intermediate strain of subgenus B. Intervirology 26: 202–206
Aird F, King JJ, Younghusband HB (1983) Identification of a new strain of adenovirus type 2 by restriction endonuclease analysis. Gene 22: 133–134
Broker TR (1980) Nucleotide sequence and analysis of group-C adenoviruses. In: Tooze J (ed) DNA tumor viruses. Molecular biology of tumor viruses, 2nd edn, part 2 J. Cold Spring Harbor Laboratory, New York, pp 923–935
Bruckova M, Wadell G, Syrucek L, Kunzova L (1980) An outbreak of respiratory disease due to a type 5 adenovirus identified a genotype 5a. Acta Virol 24: 161–165
De Jong JC, Wigand R, Kidd AH, Wadell G, Kapsenberg JG, Muzerie CJ, Wermenbol AG, Firtzlaff RG (1983) Candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool. J Med Virol 11: 215–231
Fife KH, Ashley R, Corey L (1985) Isolation and characterization of six new genome types of human adenovirus types 1 and 2. J Clin Microbiol 21: 20–23
Fox JP, Brandt CD, Wassermann FE, Hall CE, Spigland I, Kogon A, Elveback LR (1969) The virus watch program: a continuing surveillance of viral infections in metropolitan New York families. VI. Observations of adenovirus infections: virus excretion patterns, antibody response, efficiency of surveillance, patterns of infection, and relation to illness. Am J Epidemiol 89: 25–50
Green M, Mackey JK, Wold WSM, Rigden P (1979) Thirtyone human adenovirus serotypes (Ad1–AD31) form five groups (A–E) based upon DNA genome homologies. Virology 93: 481–492
Grundmann M (1988) Grenomtyp-Analyse von Adenovirusisolaten eines Jahres im Raum Hannover. Doktor-Dissertation, Homburg (Saar)
Kaier B, Wigand R (1986) Antigenic homogeneity of adenovirus types 1, 2, 5, and 6. J Med Virol 18: 283–287
Li QG, Wadell G (1986) Analysis of 15 different genome types of adenovirus type 7, isolated on five continents. J Virol 60: 331–335
Li QG, Wadell G (1988) Comparison of 17 genome types of adenovirus type 3 identified among strains recovered from six continents. J Clin Microbiol 26: 1009–1015
Niel C, D'Halluin JC (1984) Restriction maps of human adenovirus types 2, 5, and 3 for BstEII, MluI, NdeI, NruI, and SfiI. Gene 31: 305–308
Roberts RJ, O'Neil KE, Yen CT (1984) DNA sequences from the adenovirus 2 genome. J Biol Chem 259: 13968–13975
Schmitz H, Wigand R, Heinrich W (1983) Worldwide epidemiology of human adenovirus infections. Am J Epidemiol 117: 455–466
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517
Tibbetts C (1977) Physical organization of subgroup B human adenovirus genomes. J Virol 24: 564–579
Van der Avoort HGAM, Adrian T, Wigand R, Wermenbol AG, Zomerdijk TPL, de Jong JC (1986) Molecular epidemiology of adenovirus type 21 in The Netherlands and the Federal Republic of Germany from 1960 to 1985. J Clin Microbiol 24: 1084–1088
Wadell G, Varsanyi TM (1978) Demonstration of three different subtypes of adenovirus type 7 by DNA restriction site mapping. Infect Immun 21: 238–246
Wadell G, Varsanyi T, Lord A, Sutton RNP (1980) Epidemic outbreaks of adenovirus 7 with special reference to the pathogenicity of adenovirus genome type 7b. Am J Epidemiol 112: 619–628
Webb DH, Shields AF, Fife KH (1987) Genomic variation of adenovirus type 5 isolates recovered from bone marrow transplant recipients. J Clin Microbiol 25: 305–308
Wigand R, Adrian T (1986) Classification and epidemiology of adenoviruses. In: Doerfler W (ed) Adenovirus DNA. The viral genome and its expression. Martinus Nijhoff, Boston, pp 409–441
Wigand R, Bartha A, Dreizin RS, Esche H, Ginsberg HS, Green M, Hierholzer JC, Kalter SS, McFerran JB, Pettersson U, Russell WC, Wadell C (1982) Adenoviridae: second report. Intervirology 18: 169–176
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Adrian, T., Wigand, R., Knocke, K.W. et al. Genome type analysis of adenoviruses: isolates from one year from the Hannover area. Archives of Virology 105, 89–101 (1989). https://doi.org/10.1007/BF01311119
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DOI: https://doi.org/10.1007/BF01311119