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Effect of protein synthesis inhibitors on the formation of crystalloid inclusions in the endoplasmic reticulum of beetroot cells

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Abstract

Thin slices of beetroot tissue develop long ER lamellae during a period of aerated washing. After 2–3 days crystalloid bodies begin to appear within the ER cisternae and these were shown to consist largely of protein.

The effect of inhibitors of RNA and protein synthesis on the formation of ER lamellae and crystals was studied, and results indicate that protein synthesis, but not synthesis of m-RNA, is essential for both processes. It is also suggested that the protein-synthesizing system associated with the ER is more stable than the cytoplasmic polyribosome system, which may require the continued production of m-RNA after slicing.

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This research was supported by grants from the Australian Research Grants Committee.

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Van Steveninck, M.E., Van Steveninck, R.F.M. Effect of protein synthesis inhibitors on the formation of crystalloid inclusions in the endoplasmic reticulum of beetroot cells. Protoplasma 73, 107–119 (1971). https://doi.org/10.1007/BF01286416

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  • DOI: https://doi.org/10.1007/BF01286416

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