Summary
A variety of agents, including metabolic inhibitors, chelators and ionophores have been applied to suspension cultures ofAcer pseudoplatanus in an attempt at inducing cup-shaped dictyosomes. Only antipyrine was effective and then only after culturing the cells prior to treatment in sucrose-deficient medium. ATP measurements suggest a potential effect of antipyrine on energy metabolism. Both the ultrastructural effects and the reduction in ATP levels through antipyrine treatment are reversible.
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Abbreviations
- DMSO:
-
dimethylsulfoxide
- DNP:
-
dinitrophenol
- EDTA:
-
ethylene diaminotetraacetic acid
- EGTA:
-
ethylene glycol-bis (2-aminoethyl)-tetraacetic acid
- GDPH:
-
glucose-6-phosphate dehydrogenase
- HIPES:
-
2-4(2-hydroxyethyl)-piperyzine-(1)-ethynsulfonic acid
- SHAM:
-
salicylhydroxamic acid
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Glas, R., Robinson, D.G. On the induction of cup-shaped dictyosomes in suspension culturedAcer pseudoplatanus . Protoplasma 113, 97–102 (1982). https://doi.org/10.1007/BF01281997
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DOI: https://doi.org/10.1007/BF01281997