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Preliminary studies on the development of a swine vesicular disease vaccine

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Summary

The work reported is concerned with the production of swine vesicular disease virus, its inactivation and the immunogenicity of vaccines prepared from it for pigs. The virus can be grown in either monolayer or suspension cultures of IB-RS-2 cells. Virus titres of 108.5 to 109.0 p.f.u./ml have been obtained regularly in both systems in approximately 24 hours. At present the monolayer system would be used for the large-scale production of virus.

Inactivation of the virus with AEI was shown to follow a first order reaction at both 26° and 37° C. Tests on large-volume samples confirmed the innocuity of virus suspensions inactivated with this agent. With BPL it was not possible to produce fluids free of infectious virus, even with concentrations as high as 0.25 per cent.

Vaccines prepared with either aluminium hydroxide and saponin or oil as adjuvants and containing the antigen from 1.0 ml of tissue culture fluid gave high-level protection against severe challenge. Lesion scores of 0.7 and 0.4 were obtained with groups of pigs given the Al(OH)3/saponin and the oil vaccines respectively, in comparison to 14.3 for a non-vaccinated control group. The immunoglobulins formed after vaccination follow the pattern of IgM and IgG development found with other picornaviruses.

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Authors and Affiliations

  1. Animal Virus Research Institute, Pirbright, England

    G. N. Mowat, M. J. Prince, R. E. Spier & R. F. Staple

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  1. G. N. Mowat
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  2. M. J. Prince
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  3. R. E. Spier
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  4. R. F. Staple
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Mowat, G.N., Prince, M.J., Spier, R.E. et al. Preliminary studies on the development of a swine vesicular disease vaccine. Archiv f Virusforschung 44, 350–360 (1974). https://doi.org/10.1007/BF01251016

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  • DOI: https://doi.org/10.1007/BF01251016

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