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Immunological properties of attenuated variants of African swine fever virus isolated in the Russian Federation

  • Veterinary Science
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Russian Agricultural Sciences Aims and scope

Abstract

The paper presents the results of adaptation of the African swine fever (ASF) virus strain Stavropol to continuous cell cultures of homologous (A4C2, A4C2/9k, PSGK-60, and PPK-66b) and heterologous (CV-1) origin in which the virus accumulated at titers of respectively 5.0–6.5 and 4.0–4.5 logHAU50/cm3. In this case, the African swine fever virus (ASFV) multiplied in the A4C2 and A4C2/9k cell cultures with manifestation of specific hemadsorption with pig erythrocytes. The ASFV culture variants of passages 24 and 33 in the A4C2/9k and passage 20 in the CV-1 cell cultures lost pathogenicity for pigs but didn’t protect them from death after challenged with a virulent virus.

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Authors and Affiliations

  1. All-Russian Veterinary Virology and Microbiology Research Institute, Vladimir oblast, 601120, Russia

    V. I. Balysheva, E. Yu. Prudnikova & V. M. Balyshev

  2. All-Russian Experimental Veterinary Research Institute, Moscow, 109482, Russia

    T. V. Galnbek

Authors
  1. V. I. Balysheva
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  2. E. Yu. Prudnikova
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  3. T. V. Galnbek
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  4. V. M. Balyshev
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Corresponding author

Correspondence to V. I. Balysheva.

Additional information

Original Russian Text © V.I. Balysheva, E.Yu. Prudnikova, T.V. Galnbek, V.M. Balyshev, 2015, published in Doklady Rossiiskoi Akademii Sel’skokhozyaistvennykh Nauk, 2015, No. 1–2, pp. 65–69.

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Balysheva, V.I., Prudnikova, E.Y., Galnbek, T.V. et al. Immunological properties of attenuated variants of African swine fever virus isolated in the Russian Federation. Russ. Agricult. Sci. 41, 178–182 (2015). https://doi.org/10.3103/S1068367415020056

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  • DOI: https://doi.org/10.3103/S1068367415020056

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