Summary
A method was developed for the purification and concentration of Sendai virus which consisted of adsorption-elution on formolized chicken erythrocytes, of a cycle of chromatography on TEAE-cellulose, and of one cycle of differential centrifugation. A comparative study for further purification of the virus in various density gradients was undertaken. Two peaks of virus particles with densities 1.20 and 1.24 g/ml were found in CsCl gradients and one peak with density 1.18 g/ml in potassium citrate gradients. A decrease of virus infectious titer during centrifugation was observed. A method for the isolation of Sendai virus ribonucleoprotein was also developed.
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Kisseljov, F.L., Mazzarelli, M., Varitch, N.L. et al. Purification of sendai virus. Archiv f Virusforschung 27, 138–151 (1969). https://doi.org/10.1007/BF01249639
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DOI: https://doi.org/10.1007/BF01249639