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Attenuation of hog cholera virus by means of continuous cell-virus propagation (CCVP) method

II. Preparatory experiments on the attenuated strain as a live vaccine

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Summary

Attenuated hog cholera MIYAGI strain (designated LOM strain) had a high antigenieity and was safely used without serum to immunize susceptible swine against hog cholera. Average yields of virus was 105 SC-PU per ml. of the cell free fluid collected from the BK monolayer culture.

Vaccine was administered by various route, i. e., subcutaneous injection, nostril instillation with hand-spray gun, aerosol or mixed with drinking water. The resistance was detected as early as 2–4 days after vaccination and maintained for more than 300 days. The LOM vaccine containing 103 SC-PU of virus could overcome the maternally transferred antibody and the resulting immunity lasted for a long period of time.

Lyophilized vaccine was stable for more than 540 days when kept at 4° C.

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Authors and Affiliations

  1. National Veterinary Assay Laboratory, Tokyo, Japan

    Usaburo Sato, Takuma Hanaki & Kenzo Nobuto

Authors
  1. Usaburo Sato
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  2. Takuma Hanaki
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  3. Kenzo Nobuto
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Sato, U., Hanaki, T. & Nobuto, K. Attenuation of hog cholera virus by means of continuous cell-virus propagation (CCVP) method. Archiv f Virusforschung 15, 113–121 (1964). https://doi.org/10.1007/BF01241427

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  • DOI: https://doi.org/10.1007/BF01241427

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