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Biological properties of a duck enteritis virus attenuated via serial passaging in chick embryo fibroblasts

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Abstract

To gain a better understanding of the genetic changes required for attenuation of duck enteritis virus (DEV), the Chinese standard challenge strain of DEV (DEV CSC) was serially passaged 80 times in chick embryo fibroblasts. We plaque-purified the virus after the 25th passage (DEV p25) and the 80th passage (DEV p80) and investigated its in vitro and in vivo properties. Average plaque sizes for DEV p25 and p80 were significantly smaller than those for their parental DEV CSC. The results from an in vivo experiment revealed that DEV p25 and p80 were avirulent in ducks and protected them from virulent DEV challenge. The complete genome sequence of DEV p80 was determined and compared with that of the parent virus. An 1801-bp deletion was identified in the genome of DEV p80, which affected the genes encoding gI and gE. Moreover, there were 11 base substitutions, which led to seven amino acid changes in open reading frames LORF9, UL51, UL9, UL7, UL4, ICP4 and US3. Further DNA sequence analysis showed that the 1801-bp deletion was also present in DEV p25. Our findings suggest that DEV gE and/or gI are nonessential for virus growth and might, as with other herpesviruses, play an important role in cell-to-cell spread and virulence. Our experiments provide more genetic information about DEV attenuation and further advance our understanding of the molecular basis of DEV pathogenesis.

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Acknowledgments

This work was supported by the Important Animal Pathogens and Biological Reference Substance Research Program (#2008FY130100) from the Chinese Ministry of Science and Technology.

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Authors and Affiliations

  1. China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Haidian District, Beijing, 100081, People’s Republic of China

    Chenghuai Yang, Junping Li, Qihong Li, Ling Li, Miao Sun, Huijiao Li & Yecai Xia

  2. Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People’s Republic of China

    Hanchun Yang

  3. Ministry of Agriculture, No. 11 Nongzhanguan Nanli, Chaoyang District, Beijing, 100125, People’s Republic of China

    Kangzhen Yu

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  1. Chenghuai Yang
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  2. Junping Li
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  3. Qihong Li
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  4. Ling Li
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  5. Miao Sun
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  9. Kangzhen Yu
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Correspondence to Hanchun Yang or Kangzhen Yu.

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Yang, C., Li, J., Li, Q. et al. Biological properties of a duck enteritis virus attenuated via serial passaging in chick embryo fibroblasts. Arch Virol 160, 267–274 (2015). https://doi.org/10.1007/s00705-014-2275-0

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  • DOI: https://doi.org/10.1007/s00705-014-2275-0

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