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Herpes antigens from hamster kidney cell-cultures in the diagnosis of primary and recurrent infections

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Summary

Large yields of potent herpes CF antigen were prepared from infected baby hamster kidney cells (BHK21-C13) grown in 80 oz. cylindrical winchester bottles rotating about their long axes at approximately 0.3 r.p.m. Crude antigen extracted from the cells with distilled water as diluent showed no significant drop in titre when retested after 3 and 6 months storage at +4° C or −70° C; antigen stored at −40° C showed a significant drop in titre together with a decrease in sensitivity.

Sera from primary cases had higher antigen requirements than sera from recurrent cases. This qualitative difference in antibody response was not related to age of patient. In both primary and recurrent cases the antibody response was mainly associated with the sedimentable material.

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Author notes

  1. W. C. Russell

    Present address: National Institute of Medical Research, Mill Hill, London

  2. P. Wildy (Member of the Medical Research Council Experimental Virus Research Unit, Glasgow)

    Present address: Department of Virology and Bacteriology, University of Birmingham, England

Authors and Affiliations

  1. Regional Virus Laboratory, Ruchill Hospital, Glasgow, N.W.

    Constance A. C. Ross, W. C. Russell & P. Wildy (Member of the Medical Research Council Experimental Virus Research Unit, Glasgow)

  2. Institute of Virology, Glasgow, Scotland

    Constance A. C. Ross, W. C. Russell & P. Wildy (Member of the Medical Research Council Experimental Virus Research Unit, Glasgow)

Authors
  1. Constance A. C. Ross
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  2. W. C. Russell
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  3. P. Wildy
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Ross, C.A.C., Russell, W.C. & Wildy, P. Herpes antigens from hamster kidney cell-cultures in the diagnosis of primary and recurrent infections. Archiv f Virusforschung 15, 58–66 (1964). https://doi.org/10.1007/BF01241421

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  • DOI: https://doi.org/10.1007/BF01241421

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