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Colorimetric determination of pyridoxine in pharmaceutical preparations

Kolorimetrische Bestimmung von Pyridoxin in pharmazeutischen Präparaten

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Summary

The USP method for the determination of pyridoxine hydrochloride in pharmaceutical preparations has been modified to increase the stability of the colour, the speed of determination and the sensitivity. The reaction is carried out in propan-2-ol medium instead of water triethanolamine is used as the buffer, and iodine is used as oxidant when necessary. The colour is stable for about 45 min, the sensitivity of the reaction is greatly increased and the method takes only about a fifth of the time of the USP method.

Zusammenfassung

Die USP-Methode zur Bestimmung von Pyridoxinhydrochlorid in pharmazeutischen Präparaten wurde modifiziert, um die Beständigkeit der Farbe, die Geschwindigkeit der Bestimmung und deren Empfindlichkeit zu verbessern. Statt in Wasser wird die Reaktion in Propan-2-ol durchgeführt, wobei Triethanolamin als Puffer und Jod — wenn nötig — als Oxydationsmittel verwendet werden. Die Farbe ist ungefähr 45 min beständig, die Empfindlichkeit der Reaktion stark verbessert und die zur Durchführung der Methode erforderliche Zeit beträgt etwa ein Fünftel im Vergleich zur USP-Methode.

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Authors and Affiliations

  1. Central Medical Laboratories of the Egyptian Armed Forces, Cairo, Egypt

    Abdel-Fattah A. Moussa

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  1. Abdel-Fattah A. Moussa
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Moussa, AF.A. Colorimetric determination of pyridoxine in pharmaceutical preparations. Mikrochim Acta 77, 169–174 (1982). https://doi.org/10.1007/BF01237804

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  • DOI: https://doi.org/10.1007/BF01237804

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