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Determination of micro-quantities of haemoglobin in serum

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Summary

A micromethod for the determination of haemoglobin in plasma is described. It utilises the peroxidase activity of haemoglobin with CPZ as H-donor, and is sensitive to 3μg of haemoglobin per sample. Effects of substrates and reaction conditions are reported. The method can also be used for the quantitative and qualitative estimation of urine haem proteins.

Zusammenfassung

Eine Mikromethode zur Bestimmung von Hämoglobin in Plasma wurde beschrieben. Sie verwendet die Peroxydaseaktivität des Hämoglobins mit Chlorpromazin als Wasserstoffdonator und spricht auf 3μg Hämoglobin je Probe an. Der Einfluß des Substrates und der Arbeitsbedingungen wird angegeben. Das Verfahren kann auch zur quantitativen und qualitativen Analyse der Häm-Proteine im Harn verwendet werden.

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Authors and Affiliations

  1. Government Department of Pathology, Clinical Biochemistry Laboratories, Sepoy Lines, Singapore, 3

    Lee Kum-Tatt & Ai-Mee Ling

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  1. Lee Kum-Tatt
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  2. Ai-Mee Ling
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Kum-Tatt, L., Ling, AM. Determination of micro-quantities of haemoglobin in serum. Mikrochim Acta 57, 995–1002 (1969). https://doi.org/10.1007/BF01219242

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  • DOI: https://doi.org/10.1007/BF01219242

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