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Determination and properties of erythrocyte lipoyl dehydrogenase

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Summary

Erythrocyte lipoyl dehydrogenase activity has been determined using the Pd(II)-1,3-bis(2′-pyridyl)1,2-diaza-prop-2-ene colorimetric method. Properties of the red cell enzyme are described and normal values for erythrocyte lipoyl dehydrogenase are given. The possible role of this enzyme in the erythrocytes is discussed.

Zusammenfassung

Die Aktivität der Liponsäure-Dehydrogenase aus Erythrozyten wurde unter Verwendung der kolorimetrischen Methode mit Pd(II)-1,3-bis(2-pyridyl)1,2-diaza-prop-2-en bestimmt. Die Eigenschaften des Fermentes aus roten Blutzellen wurden beschrieben und Normalwerte angegeben. Die Bedeutung des Enzyms für den Stoffwechsel der Erythrozyten wurde erörtert.

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Abbreviations

EDTA:

ethylenediamino tetraacetic acid

Lip S2 :

DL-6,8-thioctic acid

Lip(SH)2 :

DL-6,8-thioctic acid, reduced form

References

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Author notes

  1. Ai-Mee Sect & Kum-Tatt Lee

    Present address: Singapore Institute of Standards and Industrial Research, 179 River Valley Road, Singapore 6, Republic of Singapore

Authors and Affiliations

  1. Government Department of Pathology, Clinical Biochemistry Laboratories, Outram Road, Singapore 3, Republic of Singapore

    Ai-Mee Sect & Kum-Tatt Lee

Authors
  1. Ai-Mee Sect
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  2. Kum-Tatt Lee
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Sect, AM., Lee, KT. Determination and properties of erythrocyte lipoyl dehydrogenase. Mikrochim Acta 64, 407–412 (1975). https://doi.org/10.1007/BF01219206

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  • DOI: https://doi.org/10.1007/BF01219206

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